tailieunhanh - Báo cáo khoa học " Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids "

Shiitake mushroom contains several therapeutic actions such as antioxidant and antimicrobial properties, carried by the diversity of its components. In the present work, extracts from shiitake mushroom were obtained using different extraction techniques: high-pressure operations and low-pressure methods. The high-pressure technique was applied to obtain shiitake extracts using pure CO2 and CO2 with co-solvent in pressures up to 30 MPa. Organic solvents such as n-hexane, ethyl acetate and dichloromethane were furthermore used to produce the shiitake extracts in low-pressure extraction process. The different extraction procedures were evaluated for antioxidant activity by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) essays and the results compared with data from Folin–Denis method, used to measure. | ELSEVIER Available online at ScienceDirect JOURNAL OF FOOD ENGINEERING Journal of Food Engineering 80 2007 631-638 locate jfoodeng Antioxidant and antimicrobial activities of shiitake Lentinula edodes extracts obtained by organic solvents and supercritical fluids Cintia Sorane Good Kitzberger a Artur Smania Jr. b Rozangela Curi Pedrosa c Sandra Regina Salvador Ferreira a a Chemical and Food Engineering Department - Federal Universtity of Santa Catarina - Laboratory of Thermodynamics and Supercritical Fluid Extraction LATESC EQA -UFSC CP 476 CEP 88040-900 Florianopolis SC Brazil b Microbiology and Parasitology Department - UFSC Brazil c Biochemistry Department - UFSC Brazil Received 10 March 2006 received in revised form 20 May 2006 accepted 16 June 2006 Available online 17 August 2006 Abstract Shiitake mushroom contains several therapeutic actions such as antioxidant and antimicrobial properties carried by the diversity of its components. In the present work extracts from shiitake mushroom were obtained using different extraction techniques high-pressure operations and low-pressure methods. The high-pressure technique was applied to obtain shiitake extracts using pure CO2 and CO2 with co-solvent in pressures up to 30 MPa. Organic solvents such as n-hexane ethyl acetate and dichloromethane were furthermore used to produce the shiitake extracts in low-pressure extraction process. The different extraction procedures were evaluated for antioxidant activity by 2 2-diphenyl-1-picryl-hydrazyl-hydrate DPPH essays and the results compared with data from Folin-Denis method used to measure the total phenolic content. Antimicrobial activities of the extracts were also subjected to preliminary screening against four strains of bacteria and one fungal strain using agar dilution method. The results indicate that the fractions obtained with CO2 using ethanol as co-solvent at 40 C 20 MPa and 15 EtOH and for dichloromethane in low-pressure .