tailieunhanh - Báo cáo khoa học " Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris "

The water soluble crude polysaccharide obtained from cultured mycelia of Cordyceps militaris (CPM) by hot water extraction followed by ethanol precipitation was fractionated by DEAE cellulose and Sepharose CL-6B column chromatography. This fractionation process resulted in four polysaccharide fractions that were termed CPMN Fr I, CPMN Fr II, CPMN Fr III, and CPMN Fr IV. Of these fractions, CPMN Fr III was able to upregulate the functional events mediated by activated macrophages, such as production of nitric oxide (NO) and expression of cytokines (IL-1b and TNF-a). Its structural characteristics were investigated by a combination of chemical and instrumental analyses, including methylation, reductive cleavage, acetylation, Fourier transform infrared. | Carbohydrate Polymers 80 2010 1011-1017 Contents lists available at ScienceDirect Carbohydrate Polymers journal homepage locate carbpol Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris Jong Seok Lee Jeong Seok Kwon Jong Seok Yun Jung Woon Pahk Won Cheol Shin Shin Young Lee Eock Kee Hong Department of Bioengineering and Technology Kangwon National University Chuncheon 200-701 Republic of Korea ARTICLE INFO ABSTRACT Article history Received 1 July 2009 Received in revised form 17 December 2009 Accepted 7 January 2010 Available online 15 January 2010 Keywords Cordyceps militaris Immunostimulating polysaccharide Macrophage activation Random coil conformation The water soluble crude polysaccharide obtained from cultured mycelia of Cordyceps militaris CPM by hot water extraction followed by ethanol precipitation was fractionated by DEAE cellulose and Sepharose CL-6B column chromatography. This fractionation process resulted in four polysaccharide fractions that were termed CPMN Fr I CPMN Fr II CPMN Fr III and CPMN Fr IV. Of these fractions CPMN Fr III was able to upregulate the functional events mediated by activated macrophages such as production of nitric oxide NO and expression of cytokines IL-1P and TNF-a . Its structural characteristics were investigated by a combination of chemical and instrumental analyses including methylation reductive cleavage acetylation Fourier transform infrared spectroscopy FT-IR and gas chromatography-mass spectrometry GC-MS . Results indicate that CPMN Fr III was a high molecular mass polysaccharide with a random coil conformation of the p-1 4-branched-p-1 6-galactoglucomannan. 2010 Elsevier Ltd. All rights reserved. 1. Introduction In recent years many natural polysaccharides and polysaccha-ride-protein complexes isolated from mushrooms have been used as therapeutic agents Novak Vetvicka 2008 . Among them Cordyceps militaris an entomopathogenic fungus belonging