tailieunhanh - Báo cáo khoa học " Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake "

In this study, structural features of Tricholoma matsutake polysaccharide (TMP-A) were investigated by a combination of infrared (IR) spectra, gas chromatography–mass spectrometry (GC–MS), nuclear magnetic resonance (NMR) spectroscopy. The results indicated that TMP-A had a backbone of 1,4- - d-glucopyranose residue which branches at O-6 based on the experimental results. The branches were mainly composed of an (1→3)- -d-galactopyranose residue, and terminated with -d-xylopyranose residue. The antioxidant activity of TMP-A was evaluated with several biochemical methods, including DPPH− radical scavenging, hydrogen peroxide scavenging, superoxide anion radical scavenging. The results indicated that TMP-A showed strong antioxidant. In the in vitro antioxidant assay by MTT method, TMP-A could attenuate PC12 cell damage. | International Journal of Biological Macromolecules 47 2010 271-275 Contents lists available at ScienceDirect International Journal of Biological Macromolecules journal homepage locate ijbiomac Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake Xiang Ding3 1 Jie Tang3 1 Mei Caob 1 Chun-xiao Guoa Xia Zhanga Jing Zhonga Jie Zhanga Qun Suna Su Fenga Zhi-rong Yanga Jian Zhaoa b a Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education College of Life Sciences Sichuan University Chengdu 610064 PR China b Key Laboratory of Sichuan Academy of Medical Sciences Sichuan Provincial People s Hospital Chengdu 610072 PR China ARTICLE INFO ABSTRACT Article history Received 20 March 2010 Received in revised form 17 April 2010 Accepted 19 April 2010 Available online 27 April 2010 Keywords Polysaccharide structure Antioxidant assay Tricholoma matsutake In this study structural features of Tricholoma matsutake polysaccharide TMP-A were investigated by a combination of infrared IR spectra gas chromatography-mass spectrometry GC-MS nuclear magnetic resonance NMR spectroscopy. The results indicated that TMP-A had a backbone of 1 4-p-D-glucopyranose residue which branches at O-6 based on the experimental results. The branches were mainly composed of an 1 3 -a-D-galactopyranose residue and terminated with a-D-xylopyranose residue. The antioxidant activity of TMP-A was evaluated with several biochemical methods including DPPH- radical scavenging hydrogen peroxide scavenging superoxide anion radical scavenging. The results indicated that TMP-A showed strong antioxidant. In the in vitro antioxidant assay by MTT method TMP-A could attenuate PC12 cell damage significantly caused by hydrogen peroxide. 2010 Elsevier . All rights reserved. 1. Introduction Oxidation is essential to many organisms for the production of energy to fuel biological processes. However the uncontrolled .