tailieunhanh - Báo cáo khoa học " In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes "

Lentinula edodes, known as ‘‘shiitake’’ is one of the widely used medicinal mushrooms in the Orient. Antitumour activity of extracts of this mushroom has been widely demonstrated in animals and humans. However, this activity was shown to be host mediated and not by direct cytotoxic activity to cancer cells. This study demonstrates cytotoxic and cell growth inhibitory (cytostatic) effect of aqueous extracts of the mushroom on MCF-7 human breast adenocarcinoma cell line using an MTT cytotoxicity assay. Such effect was demonstrated with fruit body and mycelial extracts, the difference being that there was no significant suppression on normal cells with the latter. Furthermore mycelial extracts did. | Available online at ScienceDirect Phytomedicine 15 2008 512-519 Phytomedicine phymed In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes CTc VQ Ỉ11 Ha c Q cb A rn nn rrlnila A PhlllT WXr Ml Q ci CC f T Pro f ciT1Ỉ cb . Israilides D. Kletsas D. Aiapoglou A. Philippoussis H. Piatsillis A. Ebringerovad V. Hnbalovae . Hardingf aBiotechnology Laboratory National Agricultural Research Foundation NAGREF 1 Sof. Venizelou St. 14123 Lycovrissi Athens Greece bLaboratory of Cell Proliferation Ageing National Center of Scientific Research . Demokritos Institute of Biology 15310 Athens Greece CNAGREF Institute of Agricultural Engineering Laboratory of Edible Fungi 61 Democratias St. 13561 Ag. Anargyri Athens Greece dInstitute of Chemistry Slovak Academy of Sciences Dúbravská cesta 9 845 38 Bratislava Slovakia National Institute of Public Health Srobarova 48 100 42 Prague Czech Republic f University of Nottingham MCMH School of Biosciences Sutton Bonington Leicestershire LE 12 5RD UK Abstract Lentinula edodes known as shiitake is one of the widely used medicinal mushrooms in the Orient. Antitumour activity of extracts of this mushroom has been widely demonstrated in animals and humans. However this activity was shown to be host mediated and not by direct cytotoxic activity to cancer cells. This study demonstrates cytotoxic and cell growth inhibitory cytostatic effect of aqueous extracts of the mushroom on MCF-7 human breast adenocarcinoma cell line using an MTT cytotoxicity assay. Such effect was demonstrated with fruit body and mycelial extracts the difference being that there was no significant suppression on normal cells with the latter. Furthermore mycelial extracts did not induce any cytostatic effect in both cancer and normal cell lines based on a DNA synthesis assay. The significant suppression of the proliferation of cancer cells was reflected by the comparatively low IC50 values .