tailieunhanh - Báo cáo khoa học " Isolation, composition analysis, antioxidant activity and cytotoxicity of selenium-enriched polysaccharide fractions isolated from Lentinula edodes mycelial cultures "

Polysaccharide fractions isolated from Lentinula edodes fruitbodies and mycelium demonstrate strong immunomodulating, antioxidant and anticancer activity. We hypothesized, that polysaccharide fractions enriched in selenium would possess higher biological activity than non-enriched fractions, currently used to treat cancer. The first goal of this study was to isolate selenium (Se)-enriched polysaccharide fractions of different molecular weight from L. edodes mycelium cultivated under the submerged conditions in Se-enriched medium, and to investigate their monosaccharide composition | ABSTRACTS New Biotechnology Volume 25S September 2009 I. Isolation composition analysis antioxidant activity and cytotoxicity of selenium-enriched polysaccharide fractions isolated from Lentinula edodes mycelial cultures J. Turlo1 B. Gutkowska1 F. Herold1 M. Cieslak2 J. Kazmierczak-Baranska2 1 Medical University of Warsaw Warsaw Poland 2 Centre of Molecular and Macromolecular Studies Polish Academy of Sciences Lodz Poland Polysaccharide fractions isolated from Lentinula edodes fruitbodies and mycelium demonstrate strong immunomodulating antioxidant and anticancer activity. We hypothesized that polysaccharide fractions enriched in selenium would possess higher biological activity than non-enriched fractions currently used to treat cancer. The first goal of this study was to isolate selenium Se -enriched polysaccharide fractions of different molecular weight from L. edodes mycelium cultivated under the submerged conditions in Se-enriched medium and to investigate their monosaccharide composition. The second objective was to determine whether there is relationship between the Se-content in polysaccharide fractions and their antioxidant activity. Finally we tested Se-enriched polysaccharides for their cytotoxic properties in HeLa cervix carcinoma and HUVEC normal cells. All results regarding Se-enriched polysaccharides were compared with that of reference polysaccharide fractions extracted from mycelium not enriched in Se. Concentration of Se in the Se-enriched mycelium and in the polysaccharide fractions was determined by reversed-phase high-performance liquid chromatography RP-HPLC . Total selenium amount in the L. edodes mycelium cultivated in Se-enriched 20 ppm medium was 748 g g. About 13 of total mycelial selenium was combined to the polysaccharide fractions. Four Se-enriched polysaccharide fractions were isolated from the mycelium and from the culture medium. Monosaccharide composition of selenated polysaccharides was determined after hydrolysis performed .

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• Isolation
• composition analysis
• antioxidant activity
• cytotoxicity
• selenium enriched
• mycelial cultures
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