tailieunhanh - Báo cáo khoa học " Antimicrobial properties of shiitake mushrooms (Lentinula edodes) "

Antimicrobial properties of shiitake mushrooms (Lentinula edodes) Sir, The shiitake mushroom, Lentinula edodes (Berk.) Sing, is a culinary delicacy and has traditionally been used as medicine in Asia dating back over 2000 years. Shiitake mushrooms contain protein, lipids (primarily linoleic acid), carbohydrates, fibre, minerals, vitamins B1, B2 and C, and ergosterol, the D provitamin [1]. One of the major compounds isolated from the shiitake mushroom is lentinan [(C6 H10 O5 )n ], a polysaccharide with a mean molecular mass of 500 kDa which acts as an activator for macrophage Tlymphocytes and other immune effector cells that modulate the release of. | International journal ofAntimicrobial Agents 33 2009 591-592 Contents lists available at ScienceDirect International Journal of Antimicrobial Agents journal homepage http locate ijantimicag Antimicrobial Agents Letter to the Editor Antimicrobial properties of shiitake mushrooms Lentinula edodes Sir The shiitake mushroom Lentinula edodes Berk. Sing is a culinary delicacy and has traditionally been used as medicine in Asia dating back over 2000 years. Shiitake mushrooms contain protein lipids primarily linoleic acid carbohydrates fibre minerals vitamins B1 B2 and C and ergosterol the D provitamin 1 . One of the major compounds isolated from the shiitake mushroom is lentinan CgH10O5 n a polysaccharide with a mean molecular mass of 500kDa which acts as an activator for macrophage T-lymphocytes and other immune effector cells that modulate the release of cytokines which in turn may account for its indirect antitumour and antimicrobial properties. In this study a known quantity of shiitake freeze-dried powder was suspended in an equal weight volume of sterile w v peptone saline PS CM0733 Oxoid Ltd. Basingstoke UK and filter-sterilised through a rm syringe filter Millipore UK Ltd. Watford UK before microbiological challenge. Sterile PS and antibiotic susceptibility discs containing 5 Tg of ciprofloxacin MAST Diagnostics Ltd. Bootle UK were employed as negative and positive controls respectively. All test organisms Table 1 were cultured on Columbia blood agar CM0331 Oxoid Ltd. supplemented with 5 v v defibrinated horse blood and incubated for 24 h at 37 C. Under aseptic conditions dilutions of each isolate were pre pared individually in PS equating to a McFarland standard ca. 106 colony-forming units mL which was then inoculated onto Mueller-Hinton agar CM0337 Oxoid Ltd. with a sterile cotton swab followed by fresh extracts 10 tL the inoculum was allowed to dry prior to incubation. The plates were examined visually and any inhibition was .