tailieunhanh - Báo cáo khoa học " Lentinula edodes enhances the biocontrol activity of Cryptococcus laurentii against Penicillium expansum contamination and patulin production in apple fruits "

Penicillium expansum is a post-harvest pathogen of apples which can produce the hazardous mycotoxin patulin. The yeast Cryptococcus laurentii (LS28) is a biocontrol agent able to colonize highly oxidative environments such as wounds in apples. In this study culture filtrates of the basidiomycete Lentinula edodes (LF23) were used to enhance the biocontrol activity of LS28. In vitro L. edodes culture filtrates improved the growth of C. laurentii and the activity of its catalase, superoxide dismutase and glutathione peroxidase, which play a key role in oxidant scavenging. In addition, LF23 also delayed P. expansum conidia germination. The biocontrol effect of LS28 used together with LF23 in wounded. | International Journal of Food Microbiology 138 2010 243-249 Contents lists available at ScienceDirect International Journal of Food Microbiology journal homepage locate ijfoodmicro Lentinula edodes enhances the biocontrol activity of Cryptococcus laurentii against Penicillium expansum contamination and patulin production in apple fruits VTnlaini a Q 7ia ira M Ppvprhpri a r P TTí llỉ a A A P lvlvT i a A rii l c p Pocci c A Pỉr p11ỉ b . loldini S. Zjalic M. Reveibeii C. Fanelli . Fabbii A. Del Fiore P. De Rossi A. Ricelli a Dip. Biologia Vegetale Università Sapienza Cristina di Svezia 24 00165 Roma Italy b Istituto di Chimica Biomolecolare-CNR Aldo Moro 5 00185 Roma Italy c Dip. Biotecnologie Agroindustria e Protezione salute-ENEA . Casaccia Via Anguillarese 301 00123 S. Maria di Galeria Roma Italy ARTICLE INFO ABSTRACT Article history Received 4 June 2009 Received in revised form 25 January 2010 Accepted 31 January 2010 Keywords Biocontrol Cryptococcus laurentii Lentinula edodes Penicillium expansum Patulin Oxidative stress Penicillium expansum is a post-harvest pathogen of apples which can produce the hazardous mycotoxin patulin. The yeast Cryptococcus laurentii LS28 is a biocontrol agent able to colonize highly oxidative environments such as wounds in apples. In this study culture filtrates of the basidiomycete Lentinula edodes LF23 were used to enhance the biocontrol activity of LS28. In vitro L. edodes culture filtrates improved the growth of C. laurentii and the activity of its catalase superoxide dismutase and glutathione peroxidase which play a key role in oxidant scavenging. In addition LF23 also delayed P. expansum conidia germination. The biocontrol effect of LS28 used together with LF23 in wounded apples improved the inhibition of P. expansum growth and patulin production in comparison with LS28 alone under both experimental and semicommercial conditions. The biocontrol effect was confirmed by a semi-quantitative PCR .

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