tailieunhanh - Báo cáo khoa học " Optimisation of extraction procedure for black fungus polysaccharides and effect of the polysaccharides on blood lipid and myocardium antioxidant enzymes activities "

Optimal conditions for the extraction of black fungus polysaccharides were 350 W, 5, 35 min and 90 ◦C, for ultrasonic power, ratio of water to sample, extraction time and extraction temperature, respectively. Gas chromatography (GC) analysis showed that black fungus polysaccharides contained glucose, xylose, mannose and ribose. Their molar percentages were , , and , respectively. FT-IR and NMR analysis showed typical chemical structure of black fungus polysaccharides. In animal experiment, high fat diet feeding for 29 days markedly reduced myocardium and blood antioxidant enzyme activities and enhanced lipid peroxidation level. Administration of black fungus polysaccharides had significantly enhanced myocardium and blood antioxidant enzyme activities and. | Carbohydrate Polymers 84 2011 1061-1068 Contents lists available at ScienceDirect Carbohydrate Polymers journal homepage locate carbpol Optimisation of extraction procedure for black fungus polysaccharides and effect of the polysaccharides on blood lipid and myocardium antioxidant enzymes activities Ma Jiangwei Qiao Zengyong Xiang Xia Department of Cardiovascular Medicine Fengxian Branch of Shanghai 6th People s Hospital Shanghai 201400 PR China ARTICLE INFO ABSTRACT Article history Received 13 October 2010 Received in revised form 14 December 2010 Accepted 22 December 2010 Available online 30 December 2010 Keywords Black fungus polysaccharides Antitumour activities Blood lipid High fat diet Cardiovascular diseases Optimal conditions for the extraction of black fungus polysaccharides were 350W 5 35 min and 90 C for ultrasonic power ratio of water to sample extraction time and extraction temperature respectively. Gas chromatography GC analysis showed that black fungus polysaccharides contained glucose xylose mannose and ribose. Their molar percentages were and respectively. FT-IR and NMR analysis showed typical chemical structure of black fungus polysaccharides. In animal experiment high fat diet feeding for 29 days markedly reduced myocardium and blood antioxidant enzyme activities and enhanced lipid peroxidation level. Administration of black fungus polysaccharides had significantly enhanced myocardium and blood antioxidant enzyme activities and reduced lipid peroxidation level in high fat mice. Our results indicated that black fungus polysaccharides could be beneficial for protection against cardiovascular diseases and its complications. 2011 Elsevier Ltd. All rights reserved. 1. Introduction Cardiovascular diseases CVDs are the leading causes of disability and death in industrialized nations and much of the developing world. Over the past three decades it has become clear that the onset and progression of atherosclerosis the .