tailieunhanh - Báo cáo y học: "Novel multiplex technology for diagnostic characterization of rheumatoid arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Novel multiplex technology for diagnostic characterization of rheumatoid arthritis. | Chandra et al. Arthritis Research Therapy 2011 13 R102 http content 13 3 R102 RESEARCH ARTICLE Open Access Novel multiplex technology for diagnostic characterization of rheumatoid arthritis 3 4 Piyanka E Chandra 1 Jeremy Sokolove 1 Berthold G Hipp Tamsin M Lindstrom 1 James T Elder John D Reveille5 Heike Eberl3 Ursula Klause3 and William H Robinson1 2 Abstract Introduction The aim of this study was to develop a clinical-grade automated multiplex system for the differential diagnosis and molecular stratification of rheumatoid arthritis RA . Methods We profiled autoantibodies cytokines and bone-turnover products in sera from 120 patients with a diagnosis of RA of 6 months duration as well as in sera from 27 patients with ankylosing spondylitis 28 patients with psoriatic arthritis and 25 healthy individuals. We used a commercial bead assay to measure cytokine levels and developed an array assay based on novel multiplex technology Immunological Multi-Parameter Chip Technology to evaluate autoantibody reactivities and bone-turnover markers. Data were analyzed by Significance Analysis of Microarrays and hierarchical clustering software. Results We developed a highly reproducible automated multiplex biomarker assay that can reliably distinguish between RA patients and healthy individuals or patients with other inflammatory arthritides. Identification of distinct biomarker signatures enabled molecular stratification of early-stage RA into clinically relevant subtypes. In this initial study multiplex measurement of a subset of the differentiating biomarkers provided high sensitivity and specificity in the diagnostic discrimination of RA Use of 3 biomarkers yielded a sensitivity of and a specificity of and use of 4 biomarkers a sensitivity of and a specificity of . Conclusions The multiplex biomarker assay described herein has the potential to diagnose RA with greater sensitivity and specificity than do current clinical

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