tailieunhanh - Báo cáo y học: "Molecular imaging of transcriptional regulation during inflammationƠ"

Tham khảo luận văn - đề án 'báo cáo y học: "molecular imaging of transcriptional regulation during inflammationơ"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Kielland and Carlsen Journal of Inflammation 2010 7 20 http content 7 1 20 JOURNAL OF INFLAMMATION REVIEW Open Access Molecular imaging of transcriptional regulation during inflammation Anders Kielland and Harald Carlsen Abstract Molecular imaging enables non-invasive visualization of the dynamics of molecular processes within living organisms in vivo. Different imaging modalities as MRI SPECT PET and optic imaging are used together with molecular probes specific for the biological process of interest. Molecular imaging of transcription factor activity is done in animal models and mostly in transgenic reporter mice where the transgene essentially consists of a promoter that regulates a reporter gene. During inflammation the transcription factor NF-kB is widely involved in orchestration and regulation of the immune system and almost all imaging studies in this field has revolved around the role and regulation of NF-kB. We here present a brief introduction to experimental use and design of transgenic reporter mice and a more extensive review of the various studies where molecular imaging of transcriptional regulation has been applied during inflammation. Introduction Historically conventional imaging techniques as radiography computed tomography ultrasonography and magnetic resonance imaging MRI were developed to visualize anatomical properties and changes for diagnostic purposes. Molecular imaging which has emerged as a new discipline during the last decade attempts to visualize functional properties. European Society for Molecular Imaging defines molecular imaging as the characterization of the dynamics of the molecular processes in the living organisms in vivo. The imaging modalities in molecular imaging are SPECT and PET that detect Ỵ- and p- radiation MRI that detect differences in relaxation time and optical imaging that mainly record luminescent and fluorescent light 1 . Essentially there are two types of molecular methods used in

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