tailieunhanh - Báo cáo y học: "In vitro anti-inflammatory and anti-coagulant effects of antibiotics towards Platelet Activating Factor and thrombin"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: In vitro anti-inflammatory and anti-coagulant effects of antibiotics towards Platelet Activating Factor and thrombin. | Tsoupras et al. Journal of Inflammation 2011 8 17 http content 8 1 17 JOURNAL OF INFLAMMATION RESEARCH Open Access In vitro anti-inflammatory and anti-coagulant effects of antibiotics towards Platelet Activating Factor and thrombin 1 2 2 2 2 Alexandros B Tsoupras Maria Chini Nickolaos Tsogas Athina Lioni George Tsekes Constantinos A Demopoulos 1 and Marios C Lazanas2 Abstract Background Sepsis is characterized as a systemic inflammatory response that results from the inability of the immune system to limit bacterial spread during an ongoing infection. In this condition the significant mediator of inflammation Platelet Activating Factor PAF and the coagulant factor thrombin are implicated. In animal models treatment with PAF-antagonists or co-administration of antibiotics with recombinant-PAF-Acetylhydrolase rPAF-AH have exhibited promising results. In order to examine the putative anti-inflammatory and or antithrombotic interactions between antibiotic treatment used in sepsis with PAF and or thrombin we studied the in vitro effects of these compounds towards PAF or and thrombin related activities and towards PAF basic metabolic enzymes. Methods We assessed the inhibitory effect of these drugs against PAF or thrombin induced aggregation on washed rabbit platelets WRPs or rabbit Platelet Reach Plasma rPRP by evaluating their IC50 values. We also studied their effect on Cholinephosphotransferase of PAF PAF-CPT Lyso-PAF-Acetyltransferase Lyso-PAF-AT of rabbit leukocytes RLs as well as on rabbit plasma-PAF-AH the key enzymes of both de novo remodelling PAF biosynthesis and PAF degradation respectively. Results Several antibiotics inhibited PAF-induced platelet aggregation of both WRPs and rPRP in a concentration-depended manner with clarithromycin azithromycin and amikacin exhibiting the higher inhibitory effect while when combined they synergistically inhibited PAF. Higher concentrations of all antibiotics tested were needed in order to .