tailieunhanh - Báo cáo y học: " Regional differences in the pattern of airway remodeling following chronic allergen exposure in mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:: " Regional differences in the pattern of airway remodeling following chronic allergen exposure in mice. | Respiratory Research BioMed Central Research Regional differences in the pattern of airway remodeling following chronic allergen exposure in mice Jeremy A Hirota Russ Ellis and Mark D Inman Open Access Address Firestone Institute for Respiratory Health Department of Medicine McMaster University Hamilton Ontario Canada Email Jeremy A Hirota - hirotaja@ Russ Ellis - ellisr@ Mark D Inman - inmanma@ Corresponding author Published 21 September 2006 Received 19 July 2006 Respiratory Research 2006 7 120 doi 1465-9921-7-120 Accepted 21 September 2006 This article is available from http content 7 1 120 2006 Hirota et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Airway remodeling present in the large airways in asthma or asthma models has been associated with airway dysfunction in humans and mice. It is not clear if airways distal to the large conducting airways have similar degrees of airway remodeling following chronic allergen exposure in mice. Our objective was to test the hypothesis that airway remodeling is heterogeneous by optimizing a morphometric technique for distal airways and applying this to mice following chronic exposure to allergen or saline. Methods In this study BALB c mice were chronically exposed to intranasal allergen or saline. Lung sections were stained for smooth muscle collagen and fibronectin content. Airway morphometric analysis of small 0-50000 pm2 medium 50000 pm2-175000 pm2 and large 175000 pm2 airways was based on quantifying the area of positive stain in several defined sub-epithelial regions of interest. Optimization of this technique was based on calculating sample sizes required to detect differences between