tailieunhanh - Báo cáo y học: " Open Access A key role for STIM1 in store operated calcium channel activation in airway smooth muscle"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Open Access A key role for STIM1 in store operated calcium channel activation in airway smooth muscle. | Respiratory Research BioMed Central Research Open Access A key role for STIMI in store operated calcium channel activation in airway smooth muscle Samantha E Peel Bo Liu and Ian P Hall Address Division of Therapeutics and Molecular Medicine University Hospital Queens Medical Centre Nottingham UK Email Samantha E Peel - msxsp@ Bo Liu - Ian P Hall - Corresponding author Published 20 September 2006 Received 20 June 2006 Respiratory Research 2006 7 119 doi 1465-9921-7-119 Accepted 20 September 2006 This article is available from http content 7 1 1 19 2006 Peel et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Control of cytosolic calcium plays a key role in airway myocyte function. Changes in intracellular Ca2 stores can modulate contractile responses modulate proliferation and regulate synthetic activity. Influx of Ca2 in non excitable smooth muscle is believed to be predominantly through store operated channels SOC or receptor operated channels ROC . Whereas agonists can activate both SOC and ROC in a range of smooth muscle types the specific trigger for SOC activation is depletion of the sarcoplasmic reticulum Ca2 stores. The mechanism underlying SOC activation following depletion of intracellular Ca2 stores in smooth muscle has not been identified. Methods To investigate the roles of the STIM homologues in SOC activation in airway myocytes specific siRNA sequences were utilised to target and selectively suppress both STIM1 and STIM2. Quantitative real time PCR was employed to assess the efficiency and the specificity of the siRNA mediated knockdown of mRNA. Activation of SOC was investigated by both .