tailieunhanh - Báo cáo y học: " Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM. | Respiratory Research BioMed Central Research Open Access Caveolin-I and -2 in airway epithelium expression and in situ association as detected by FRET-CLSM Gabriela Krasteva1 Uwe Pfeil1 Marek Drab2 Wolfgang Kummer1 and Peter Konig 1 Address 1Institut fur Anatomie und Zellbiologie University of Giessen Lung Center Justus-Liebig-Universitat Giessen Germany and 2Max-Planck Institute for Infection Biology Department of Molecular Biology Berlin Germany Email Gabriela Krasteva- Uwe Pfeil - Marek Drab - drab@ Wolfgang Kummer - Peter Konig - Corresponding author Published II August 2006 Received 17 April 2006 Respiratory Research 2006 7 108 doi 1465-9921-7-108 Accepted 1 1 August 2006 This article is available from http content 7 1 108 2006 Krasteva et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Caveolae are involved in diverse cellular functions such as signal transduction cholesterol homeostasis endo- and transcytosis and also may serve as entry sites for microorganisms. Hence their occurrence in epithelium of the airways might be expected but nonetheless has not yet been examined. Methods Western blotting real-time quantitative PCR analysis of abraded tracheal epithelium and laser-assisted microdissection combined with subsequent mRNA analysis were used to examine the expression of cav-1 and cav-2 two major caveolar coat proteins in rat tracheal epithelium. Fluorescence immunohistochemistry was performed to locate caveolae and cav-1 and -2 in the airway epithelium of rats mice and .

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