tailieunhanh - Báo cáo y học: " Human bronchial fibroblasts express the 5-lipoxygenase pathway"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Human bronchial fibroblasts express the 5-lipoxygenase pathway. | Respiratory Research BioMed Central Research Human bronchial fibroblasts express the 5-lipoxygenase pathway Anna J James John F Penrose Angelica M Cazaly Stephen T Holgate and Anthony P Sampson Open Access Address Division of Infection Inflammation and Repair IIR University of Southampton School of Medicine Southampton General Hospital Southampton SO16 6YD UK Email Anna J James - annajuliajames@ John F Penrose - jfpdoc@ Angelica M Cazaly- ac7@ Stephen T Holgate - Anthony P Sampson - Corresponding author Published 27 July 2006 Received 24 April 2006 Respiratory Research 2006 7 102 doi 1465-9921-7-102 Accepted 27 July 2006 This article is available from http content 7 1 102 2006 James et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Fibroblasts are implicated in sub-epithelial fibrosis in remodeled asthmatic airways and contribute to airway inflammation by releasing cytokines and other mediators. Fibroblast activity is influenced by members of the leukotriene family of bronchoconstrictor and inflammatory mediators but it is not known whether human bronchial fibroblasts can synthesize leukotrienes. Methods The expression of leukotriene biosynthetic enzymes and receptors was investigated in primary fibroblasts from the bronchi of normal and asthmatic adult subjects using RT-PCR Western blotting immunocytochemistry and flow cytometry. Results These techniques revealed that human bronchial fibroblasts from both subject groups constitutively express 5-lipoxygenase its activating protein FLAP the terminal enzymes leukotriene A4 hydrolase and leukotriene C4 synthase and receptors for leukotriene B4 .