tailieunhanh - Báo cáo y học: " Expression of eicosanoid receptors subtypes and eosinophilic inflammation: implication on chronic rhinosinusitis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Expression of eicosanoid receptors subtypes and eosinophilic inflammation: implication on chronic rhinosinusitis. | Respiratory Research BioMed Central Research Open Access Expression of eicosanoid receptors subtypes and eosinophilic inflammation implication on chronic rhinosinusitis Claudina Angela Pérez-Novo Cindy Claeys Paul Van Cauwenberge and Claus Bachert Address Upper Airways Research Laboratory Department of Otorhinolaryngology Ghent University Hospital De Pintelaan 85 Ghent Belgium Email Claudina Angela Pérez-Novo - Cindy Claeys - Paul Van Cauwenberge - Claus Bachert- Corresponding author Published 12 May 2006 Received 24 October 2005 Respiratory Research 2006 7 75 doi 1465-9921 -7-75 Accepted 12 May 2006 This article is available from http content 7 1 75 2006 Pérez-Novo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Eicosanoid receptors are G-protein-coupled receptors playing an important immunomodulatory role in airway diseases. However there is little information on the expression of these receptors and their link with eosinophilic inflammation in paranasal sinus diseases. We aimed with this study to investigate the tissue expression of leukotrienes and prostaglandin E2 receptors in chronic rhinosinusitis patients and the link of this regulation with eosinophilic inflammation. Methods Samples were prepared from nasal tissue of patients with chronic rhinosinusitis without nasal polyps CRS n 11 with nasal polyps CRS-NP n 13 and healthy subjects Controls n 6 . mRNA expression of CysLT1 CysLT2 BLT1 BLT2 E-prostanoid receptors EP1 EP2 EP3 EP4 and sol-IL-5Ra was determined by real-time PCR. Concentrations of PGE2 LTC4 D4 E4 LTB4 and sol-IL-5Ra were determined by ELISA and