tailieunhanh - Báo cáo khoa hoc : Mass spectrometric characterization of proteins transferred from polyacrylamide gels to membrane filters

n the 1990s, a technique was developed to transfer proteins from electro-phoresis gels onto poly(vinylidene difluoride) (PVDF) membranes, digest the proteins on the membranes with proteases such as trypsin and analyze the resulting peptides on the membranes directly by mass spectrometry to identify the proteins. | IFEBS Journal REVIEW ARTICLE Mass spectrometric characterization of proteins transferred from polyacrylamide gels to membrane filters Yoko Ino and Hisashi Hirano Advanced MedicalResearch Center Graduate Schoolof Nanobioscience Yokohama City University Japan Keywords electroblotting gel electrophoresis mass spectrometry MS protein Correspondence H. Hirano Yokohama City University Advanced Medical Research Center Fukuura 3-9 Kanazawa Yokohama 236-0004 Japan Fax 81 45 787 2787 Tel 81 45 787 2791 E-mail hirano@ Received 2 April 2011 revised 1 August 2011 accepted 11 August 2011 In the 1990s a technique was developed to transfer proteins from electrophoresis gels onto poly vinylidene difluoride PVDF membranes digest the proteins on the membranes with proteases such as trypsin and analyze the resulting peptides on the membranes directly by mass spectrometry to identify the proteins. This technique based on gel electrophoresis is particularly useful for analyzing protein isoforms splicing variants and post-translationally modified proteins. Previously the low ionization efficiency of peptides immobilized on the membranes often rendered this technique useless. However this technique has been improved by the use of PVDF membranes with a small pore size which has enabled highly efficient and effective electroblotting and mass spectrometric analyses. Here the advantage of this technique is discussed. doi Proteomic analyses begin with protein expression profiling Proteome research involves the comprehensive analysis of protein expression profiles the function and functional networks of proteins and the relationship between proteins and diseases. Upgraded protein databases and highly sensitive accurate and high-throughput analytical techniques such as mass spectrometry MS have greatly facilitated and accelerated the development of proteome research. Two standard methods the protein shotgun method 1 and the 2D electrophoresis 2DE

• Báo cáo khoa học
• Biochemistry
• molecular Biology
• biological cells
• membrane filters
• cytoplasm
• biochemical studies
• Textbook of biochemistry
• Chemical basis of life
• General metabolism
• Clinical and applied biochemistry
• Applied biochemistry
• Chemistry of lipids
• Chemistry of carbohydrates
• Overview of metabolism
• Advanced biochemistry
• Steroid hormones
• Control of gene expression
• Principles of biochemistry
• Lecture Principles of biochemistry
• Biological systems
• Biochemical compounds
• Biological macromolecules
• Chemical principles
• Ebook Principles of biochemistry
• Principles of biochemistry (4th edition)
• The glyoxylate cycle
• Fatty acid catabolism
• Electron Transfer reactions
• Evolutionary foundations
• Protein interactions modulated
• Nucleic acid structure
• Ebook Plant biochemistry
• Plant biochemistry
• Plant biochemistry (Third edition)
• Several metabolic compartments
• The plant cell mechanical
• The chlorophyll molecule
• Nodule forming bacteria
• The amino acids
• The endoplasmic reticulum
• Special topics
• Harper’s illustrated biochemistry
• Replication of informational macromolecules
• Biochemistry of extracellular
• Intracellular communication
• Illustrated biochemistry
• Biochemical case histories
• The biochemistry of aging
• Signal transduction
• Hormone action
• Clinical biochemistry
• Analytical BioChemistry
• Preparation Techniques
• Analytical Chemistry
• chemical analysis
• the overall chemical analysis
• chemical analysis of materials
• General biochemistry
• biochemistry lectures
• animal biochemistry
• biochemical
• dynamic
• static biochemical
• cell membranes
• Biotechnology Biochemistry
• Algae Anatomy
• Biotechnology
• Glaucophyta
• Endosymbiosis and Origin
• Axoneme
• Lehninger principles of biochemistry
• The foundations of biochemistry
• Structure and catalysis
• The three dimensional structure of proteins
• Carbohydrates and glycobiology
• Protein function
• BRS biochemistry molecular biology
• Fuel metabolism
• Basic principles
• Basic aspects of biochemistry
• Acid–base chemistry
• Gene expression
• Synthesis of proteins
• Properties of water
• Hydrogen bonding
• Amino acids
• Amino acid structure
• Protein purification
• Cell extract
• Protein classification
• Protein 3 Dimensional structure
• Protein folding
• Protein 3 D structure
• 3 D structure
• Oxygen binding curve
• Enzyme kinetics
• Rate constants
• Catalytic Power
• Classes of enzymes
• Inhibitor kinetics
• Enzyme regulation
• Allosteric regulation
• Enzyme action
• Mechanisms of enzyme action