tailieunhanh - Báo cáo khoa hoc : Barnase and binase: twins with distinct fates

RNases are enzymes that cleave RNAs, resulting in remarkably diverse biological consequences. Many RNases are cytotoxic. In some cases, they attack selectively malignant cells triggering an apoptotic response. | fFEBS Journal REVIEW ARTICLE Barnase and binase twins with distinct fates Vera Ulyanova Valentina Vershinina and Olga Ilinskaya Department of Microbiology Kazan Volga Region FederalUniversity Kazan Russia Keywords Bacillus bacterialRNases barnase binase biosynthesis cancer therapy cytotoxicity physiologicalrole practicalapplication RNase inhibitor Correspondence O. Ilinskaya Department of Microbiology Kazan Volga Region FederalUniversity 18 Kremlyovskaya Street Kazan 420008 Russia Fax 7843 292 7418 Tel 7843 233 7855 E-mail Received 3 March 2011 revised 29 July 2011 accepted 4 August 2011 doi RNases are enzymes that cleave RNAs resulting in remarkably diverse biological consequences. Many RNases are cytotoxic. In some cases they attack selectively malignant cells triggering an apoptotic response. A number of eukaryotic and bacterial RNase-based strategies are being developed for use in anticancer and antiviral therapy. However the physiological functions of these RNases are often poorly understood. This review focuses on the properties of the extracellular RNases from Bacillus amyloliquefac-iens barnase and Bacillus intermedius binase the characteristics of their biosynthesis regulation and their physiological role with an emphasis on the similarities and differences. Barnase and binase can be regarded as molecular twins according to their highly similar structure physical-chemical and catalytic properties. Nevertheless the life paths of these enzymes are not the same as their expression in bacteria is controlled by diverse signals. Binase is predominantly synthesized under phosphate starvation whereas barnase production is strictly dependent on the multifunctional SpoOA regulator controlling sporulation biofilm formation and cannibalism. Barnase and binase also have some distinctions in practical applications. Barnase was initially suggested to be useful in research and biotechnology as a tool for studying .