tailieunhanh - Báo cáo y học: "Underexpression of mitochondrial-DNA encoded ATP synthesis-related genes and DNA repair genes in systemic lupus erythematosus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Underexpression of mitochondrial-DNA encoded ATP synthesis-related genes and DNA repair genes in systemic lupus erythematosus. | Lee et al. Arthritis Research Therapy 2011 13 R63 http content 13 2 R63 RESEARCH ARTICLE Open Access Underexpression of mitochondrial-DNA encoded ATP synthesis-related genes and DNA repair genes in systemic lupus erythematosus Hooi-Ming Lee1 Hidehiko Sugino1 Chieko Aoki2 and Norihiro Nishimoto1 2 Abstract Introduction Systemic lupus erythematosus SLE is a prototypical autoimmune disease characterized by various systemic symptoms and multiple organ damage. We clarify biological and functional abnormalities in SLE by comparing the gene expression profiles of SLE patients with those of healthy individuals. Methods Gene expression profiles from the peripheral blood of 21 SLE patients and 45 healthy individuals were obtained using a DNA microarray. Gene ontology analysis and network pathway analysis were performed on the genes differentially expressed between SLE and healthy individuals. Results A total of 2 329 upregulated genes and 1 884 downregulated genes were differentially expressed. Gene ontology analysis revealed that the upregulated genes were classified as response to biotic stimulus genes which mainly includes genes related to immune response. Abnormalities in other categories such as cell motility and regulation of apoptosis were also revealed. Downregulated genes were mainly sorted into two gene categories sensory perception and response to radiation light. The sensory perception genes included ATPase ATPase domain-containing genes myosin-related genes and two excision repair cross-complementing genes which are involved in DNA repair. Other genes in this group - including three crystallin genes genes encoding the receptor protein for melanocyte-stimulating hormone and six mitochondrial-DNA encoded genes which are involved in ATP synthesis - were also categorized as response to radiation genes. Using network pathway analysis IL-6 transforming growth factor beta 1 TNF and hepatocyte nuclear factor 4a were found to play central roles in