tailieunhanh - Báo cáo y học: "Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus. | Lood et al. Arthritis Research Therapy 2011 13 R60 http content 13 2 R60 RESEARCH ARTICLE Open Access Protein synthesis of the pro-inflammatory S100A8 A9 complex in plasmacytoid dendritic cells and cell surface S100A8 A9 on leukocyte subpopulations in systemic lupus erythematosus 3 2 13 3 Christian Lood 1 Martin Stenstrom Helena Tydén Birgitta Gullstrand Eva Kallberg Tomas Leanderson Lennart Truedsson1 Gunnar Sturfelt2 Fredrik Ivars3 and Anders A Bengtsson2 Abstract Introduction Systemic lupus erythematosus SLE is an autoimmune disease with chronic or episodic inflammation in many different organ systems activation of leukocytes and production of pro-inflammatory cytokines. The heterodimer of the cytosolic calcium-binding proteins S100A8 and S100A9 S100A8 A9 is secreted by activated polymorphonuclear neutrophils PMNs and monocytes and serves as a serum marker for several inflammatory diseases. Furthermore S100A8 and S100A9 have many pro-inflammatory properties such as binding to Toll-like receptor 4 TLR4 . In this study we investigated if aberrant cell surface S100A8 A9 could be seen in SLE and if plasmacytoid dendritic cells pDCs could synthesize S100A8 A9. Methods Flow cytometry confocal microscopy and real-time PCR of flow cytometry-sorted cells were used to measure cell surface S100A8 A9 intracellular S100A8 A9 and mRNA levels of S100A8 and S100A9 respectively. Results Cell surface S100A8 A9 was detected on all leukocyte subpopulations investigated except for T cells. By confocal microscopy real-time PCR and stimulation assays we could demonstrate that pDCs monocytes and PMNs could synthesize S100A8 A9. Furthermore pDC cell surface S100A8 A9 was higher in patients with active disease as compared to patients with inactive disease. Upon immune complex stimulation pDCs up-regulated the cell surface S100A8 A9. SLE patients had also increased serum levels of S100A8 A9. Conclusions Patients with SLE had increased cell surface S100A8 A9 .