tailieunhanh - Báo cáo y học: "Ca2+-signaling in airway smooth muscle cells is altered in T-bet knock-out mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Ca2+-signaling in airway smooth muscle cells is altered in T-bet knock-out mice. | Respiratory Research BioMed Central Research Open Access Ca2 -signaling in airway smooth muscle cells is altered in T-bet knock-out mice Albrecht Bergner Julia Kellner Anita Kemp da Silva Fernando Gamarra and Rudolf M Huber Address Pneumology Medizinische Klinik-Innenstadt Ludwig-Maximilians-University Munich Germany Email Albrecht Bergner - Julia Kellner - Anita Kemp da Silva - Fernando Gamarra - Rudolf M Huber - huber@ Corresponding author Published 23 February 2006 Received 26 November 2005 Respiratory Research 2006 7 33 doi 1465-9921-7-33 Accepted 23 February 2006 This article is available from http content 7 1 33 2006 Bergner et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Airway smooth muscle cells ASMC play a key role in bronchial hyperresponsiveness BHR . A major component of the signaling cascade leading to ASMC contraction is calcium. So far agonist-induced Ca2 -signaling in asthma has been studied by comparing innate properties of inbred rat or mouse strains or by using selected mediators known to be involved in asthma. T-bet knock-out KO mice show key features of allergic asthma such as a shift towards TH2-lymphocytes and display a broad spectrum of asthma-like histological and functional characteristics. In this study we aimed at investigating whether Ca2 -homeostasis of ASMC is altered in T-bet KO-mice as an experimental model of asthma. Methods Lung slices of 100 to 200 pm thickness were obtained from T-bet KO- and wild-type mice. Airway contraction in response to acetylcholine ACH was measured .