tailieunhanh - Báo cáo y học: " Dysregulated expression of MIG/CXCL9, IP-10/CXCL10 and CXCL16 and their receptors in systemic sclerosis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Dysregulated expression of MIG/CXCL9, IP-10/CXCL10 and CXCL16 and their receptors in systemic sclerosis. | Rabquer et al. Arthritis Research Therapy 2011 13 R18 http content 13 1 R18 RESEARCH ARTICLE Open Access Dysregulated expression of MIG CXCL9 IP-10 CXCL10 and CXCL16 and their receptors in systemic sclerosis 1 11 1 2 Bradley J Rabquer Pei-Suen Tsou Yong Hou Eshwar Thirunavukkarasu G Kenneth Haines 3rd 1 1 3 14 15 Ann J Impens Kristine Phillips Bashar Kahaleh James R Seibold Alisa E Koch Abstract Introduction Systemic sclerosis SSc is characterized by fibrosis and microvascular abnormalities including dysregulated angiogenesis. Chemokines in addition to their chemoattractant properties have the ability to modulate angiogenesis. Chemokines lacking the enzyme-linked receptor ELR motif such as monokine induced by interferon-y IFN-y MIG CXCL9 and IFN-inducible protein 10 IP-10 CXCL10 inhibit angiogenesis by binding CXCR3. In addition CXCL16 promotes angiogenesis by binding its unique receptor CXCR6. In this study we determined the expression of these chemokines and receptors in SSc skin and serum. Methods Immunohistology and enzyme-linked immunosorbent assays ELISAs were used to determine chemokine and chemokine receptor expression in the skin and serum respectively of SSc and normal patients. Endothelial cells ECs were isolated from SSc skin biopsies and chemokine and chemokine receptor expression was determined by quantitative PCR and immunofluorescence staining. Results Antiangiogenic IP-10 CXCL10 and MIG CXCL9 were elevated in SSc serum and highly expressed in SSc skin. However CXCR3 the receptor for these chemokines was decreased on ECs in SSc vs. normal skin. CXCL16 was elevated in SSc serum and increased in SSc patients with early disease pulmonary arterial hypertension and those that died during the 36 months of the study. In addition its receptor CXCR6 was overexpressed on ECs in SSc skin. At the mRNA and protein levels CXCR3 was decreased while CXCR6 was increased on SSc ECs vs. human microvascular endothelial cells HMVECs . Conclusions