tailieunhanh - Báo cáo y học: "mPGES-1 null mice are resistant to bleomycininduced skin fibrosis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: mPGES-1 null mice are resistant to bleomycininduced skin fibrosis. | McCann et al. Arthritis Research Therapy 2011 13 R6 http content 13 1 R6 RESEARCH ARTICLE Open Access mPGES-1 null mice are resistant to bleomycin-induced skin fibrosis 1 t 2 1 1 n 2 Matthew R McCann t Roxana Monemdjou Paiisa Ghassemi-Kakioodi Hassan Fahmi Gemma Perez Qhunnvi I II I1 Yi I CHi- A Cifi3 Cl IniI Drticqr i irTỉm1 Pl IZc iiimTi4 CbriclTinhcir D rìontTMO3 I AHrTỉhTỉm3 Siiangxi Liu XU Shi wen Sunil K raiapuiam Fumiaki Kojima Ciiiistopnei P Denton David J Abiaiiam I Cl h o o o I o rpo I Po 11ort or2 I oc I io I c mffnrH4 I d3cL 1t i lcHir k sir i ccr2 Johanne Maitei Peiietiei Leslie J Cioiioid Andiew Leask Mohit Kapooi Abstract Introduction Microsomal prostaglandin E2 synthase-1 mPGES-1 is an inducible enzyme that acts downstream of cyclooxygenase COX to specifically catalyze the conveision of prostaglandin PG H2 to PGE2. mPGES-1 plays a key rale in inflammation pain and arthritis howevei the rale of mPGES-1 in fibiogenesis is laigely unknown. Herein we examine the rale of mPGES-1 in a mouse model of skin scleiodeima using mice deficient in mPGES-1. Methods Wild type WT and mPGES-1 null mice were subjected to the bleomycin model of cutaneous skin scleiodeima. mPGES-1 expressions in scleiodeima fibroblasts and in fibroblasts derived fiom bleomycin-exposed mice weie assessed by Western blot analysis. Degree of fibiosis deimal thickness inflammation collagen content and the numbei of a-smooth muscle actin a-SMA -positive cells weie deteimined by histological analyses. The quantity of the collagen-specific amino acid hydioxypioline was also measured. Results Compared to noimal skin fibroblasts mPGES-1 piotein expression was elevated in systemic sclerosis SSc fibroblasts and in bleomycin-exposed mice. Compared to WT mice mPGES-1-null mice weie resistant to bleomycin-induced inflammation cutaneous thickening collagen production and myofibroblast foimation. Conclusions mPGES-1 expression is required foi bleomycin-induced skin .