tailieunhanh - Báo cáo y học: "Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254)"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254). | Catterall et al. Arthritis Research Therapy 2010 12 R229 http content 12 6 R229 RESEARCH ARTICLE Open Access Changes in serum and synovial fluid biomarkers after acute injury NCT00332254 1 1 2 1 Jonathan B Catterall Thomas V Stabler Carl R Flannery Virginia B Kraus Abstract Introduction Acute trauma involving the anterior cruciate ligament is believed to be a major risk factor for the development of post-traumatic osteoarthritis 10 to 20 years post-injury. In this study to better understand the early biological changes which occur after acute injury we investigated synovial fluid and serum biomarkers. Methods We collected serum from 11 patients without pre-existing osteoarthritis from a pilot intervention trial 5 placebo and 6 drug treated using an intra-articular interleukin-1 receptor antagonist IL-1Ra therapy 9 of which also supplied matched synovial fluid samples at presentation to the clinic after acute knee injury mean days and at the follow-up visit for reconstructive surgery mean days . To exclude patients with preexisting osteoarthritis OA the study was limited to individuals younger than 40 years of age mean 23 with no prior history of joint symptoms or trauma. We profiled a total of 21 biomarkers 20 biomarkers in synovial fluid and 13 in serum with 12 biomarkers measured in both fluids. Biomarkers analyzed in this study were found to be independent of treatment P as measured by Mann-Whitney and two-way ANOVA. Results We observed significant decreases in synovial fluid sf biomarker concentrations from baseline to followup for sfC-Reactive protein CRP P sflubricin P and the proteoglycan biomarkers sfGlycosaminoglycan GAG P and sfAlanine-Arginine-Glycine-Serine ARGS aggrecan P . In contrast we observed significant increases in the collagen biomarkers sfC-terminal crosslinked telopeptide type II collagen CTxII P sfC1 2C P sfC-terminal crosslinked telopeptide type I .