tailieunhanh - Báo cáo y học: " Arginase strongly impairs neuronal nitric oxide-mediated airway smooth muscle relaxation in allergic asthma"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Arginase strongly impairs neuronal nitric oxide-mediated airway smooth muscle relaxation in allergic asthma. | Respiratory Research BioMed Central Research Open Access Arginase strongly impairs neuronal nitric oxide-mediated airway smooth muscle relaxation in allergic asthma Harm Maarsingh John Leusink I Sophie T Bos Johan Zaagsma and Herman Meurs Address Department of Molecular Pharmacology University Centre for Pharmacy University of Groningen Antonius Deusinglaan 1 9713 AV Groningen The Netherlands Email Harm Maarsingh - John Leusink - I Sophie T Bos - Johan Zaagsma - Herman Meurs - Corresponding author Published 12 January 2006 Received 25 October 2005 Respiratory Research 2006 7 6 doi l465-992l-7-6 Accepted l2 January 2006 This article is available from http content 7 l 6 2006 Maarsingh et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Using guinea pig tracheal preparations we have recently shown that endogenous arginase activity attenuates inhibitory nonadrenergic noncholinergic iNANC nerve-mediated airway smooth muscle relaxation by reducing nitric oxide NO production - due to competition with neuronal NOsynthase nNOS for the common substrate L-arginine. Furthermore in a guinea pig model of allergic asthma airway arginase activity is markedly increased after the early asthmatic reaction EAR leading to deficiency of agonist-induced epithelium-derived NO and subsequent airway hyperreactivity. In this study we investigated whether increased arginase activity after the EAR affects iNANC nerve-derived NO production and airway smooth muscle relaxation. Methods Electrical field stimulation EFS 150 mA 4 ms 4 s - l6 Hz -induced relaxation was measured in tracheal open-ring .