tailieunhanh - Báo cáo y học: " RFX1 regulates CD70 and CD11a expression in lupus T cells by recruiting the histone methyltransferase SUV39H1"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: RFX1 regulates CD70 and CD11a expression in lupus T cells by recruiting the histone methyltransferase SUV39H1. | Zhao et al. Arthritis Research Therapy 2010 12 R227 http content 12 6 R227 RESEARCH ARTICLE Open Access RFX1 regulates CD70 and CD11a expression in lupus T cells by recruiting the histone methyltransferase SUV39H1 Ming Zhao Xiaoyan Wu Qing Zhang Shuangyan Luo Gongping Liang Yuwen Su Yixin Tan Qianjin Lu Abstract Introduction Regulatory factor X-box 1 RFX1 can interact with DNA methyltransferase 1 DNMT1 and histone deacetylase 1 HDAC1 and RFX1 down-regulation contributes to DNA hypomethylation and histone H3 hyperacetylation at the cluster of differentiation CD 11a and CD70 promoters in CD4 T cells of patients with systemic lupus erythematosus SLE . This leads to CD11a and CD70 overexpression thereby triggering autoimmune responses. In order to provide more insight into the epigenetic mechanisms leading to the deregulation of autoimmune-related genes in SLE we asked whether RFX1 is involved in regulating histone 3 lysine 9 H3K9 trimethylation at the CD11a and CD70 promoters in SLE CD4 T cells. Methods CD4 T cell samples were isolated from 15 SLE patients and 15 healthy controls. H3K9 tri-methylation levels were measured by chromatin immunoprecipitation ChIP and real-time quantitative PCR. CD4 T cells were transfected with plasmids using the Human T cell Nucleofector Kit. RFX1 and histone methyltransferase suppressor of variegation 3-9 Drosophila homolog 1 SUV39H1 interaction was determined by co-immunoprecipation co-IP and Western blot and immunofluorescence staining. CD11a and CD70 mRNA levels were measured by real-time RT-PCR. Results H3K9 tri-methylation levels were significantly reduced within the CD11a and CD70 promoter regions in SLE CD4 T cells. RFX1 co-immunoprecipitated with SUV39H1 at the CD11a and CD70 promoters in healthy control CD4 T cells. Overexpressing or knocking-down RFX1 revealed that RFX1 expression correlated with H3K9 trimethylation levels as well as CD11a and CD70 expression levels in CD4 T cells. Conclusions RFX1 .