tailieunhanh - Báo cáo y học: "Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats". | Respiratory Research BioMed Central Research Open Access Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats Holger Garn 1 Anette Siese2 Sabine Stumpf2 Anka Wensing1 Harald Renz1 and Diethard Gemsa2 Address Department of Clinical Chemistry and Molecular Diagnostics Philipps University of Marburg Biomedical Research Center Hans-Meerwein-Str. 35043 Marburg Germany and 2Institute of Immunology Philipps University of Marburg Robert-Koch-Str. 17 35037 Marburg Germany Email Holger Garn - garn@ Anette Siese - asiese@ Sabine Stumpf - sabine@ Anka Wensing - Harald Renz - renzh@ Diethard Gemsa - gemsa@ Corresponding author Published 06 January 2006 Received 15 August 2005 Respiratory Research 2006 7 4 doi l465-992l-7-4 Accepted 06 January 2006 This article is available from http content 7 l 4 2006 Garn et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Alveolar macrophages AM are known to play an important role in the regulation of inflammatory reactions in the lung . during the development of chronic lung diseases. Exposure of rats to NO2 has recently been shown to induce a shift in the activation type of AM that is characterized by reduced TNF-a and increased IL-10 production. So far it is unclear whether a functional shift in the already present AM population or the occurrence of a new phenotypically different AM population is responsible for these observations. Methods AM from rat and mice were analyzed by flow cytometry for surface marker expression and in vivo staining with PKH26 was applied to .