tailieunhanh - Báo cáo y học: " TGF-β1 increases proliferation of airway smooth muscle cells by phosphorylation of map kinases"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" TGF-β1 increases proliferation of airway smooth muscle cells by phosphorylation of map kinases. | Respiratory Research BioMed Central Research Open Access TGF-P1 increases proliferation of airway smooth muscle cells by phosphorylation of map kinases Gang Chen and Nasreen Khalil Address Division of Respiratory Medicine Department of Medicine The University of British Columbia and the Vancouver Coastal Health Research Institute Vancouver BC V6H 3Z6 Canada Email Gang Chen - Nasreen Khalil - nkhalil@ Corresponding author Published 03 January 2006 Received 16 August 2005 Respiratory Research 2006 7 2 doi l465-992l-7-2 Accepted 03 January 2006 This article is available from http content 7 l 2 2006 Chen and Khalil licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Airway remodeling in asthma is the result of increased expression of connective tissue proteins airway smooth muscle cell ASMC hyperplasia and hypertrophy. TGF-Pl has been found to increase ASMC proliferation. The activation of mitogen-activated protein kinases MAPKs p38 ERK and JNK is critical to the signal transduction associated with cell proliferation. In the present study we determined the role of phosphorylated MAPKs in TGF-P l induced ASMC proliferation. Methods Confluent and growth-arrested bovine ASMCs were treated with TGF-Pl. Proliferation was measured by 3H -thymidine incorporation and cell counting. Expressions of phosphorylated p38 ERKl 2 and JNK were determined by Western analysis. Results In a concentration-dependent manner TGF-Pl increased 3H -thymidine incorporation and cell number of ASMCs. TGF-Pl also enhanced serum-induced ASMC proliferation. Although ASMCs cultured with TGF-Pl had a significant increase in phosphorylated p38 ERKl 2 and JNK the maximal .