tailieunhanh - Báo cáo y học: "Intraarticular injection of hyaluronan prevents cartilage erosion, periarticular fibrosis and mechanical allodynia and normalizes stance time in murine knee osteoarthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Intraarticular injection of hyaluronan prevents cartilage erosion, periarticular fibrosis and mechanical allodynia and normalizes stance time in murine knee osteoarthritis. | Plaas et al. Arthritis Research Therapy 2011 13 R46 http content 13 2 R46 RESEARCH ARTICLE Open Access Intraarticular injection of hyaluronan prevents cartilage erosion periarticular fibrosis and mechanical allodynia and normalizes stance time in murine knee osteoarthritis 1112 3 Anna Plaas Jun Li Julie Riesco Rosalina Das John D Sandy and Andrew Harrison Abstract Introduction Intraarticular hyaluronan HA is used clinically for symptomatic relief in patients with knee osteoarthritis OA however the mechanism of action is unclear. In this study we examined the effects of a single injection of HA on joint tissue pathology mechanical allodynia and gait changes measured by stride times in a murine model of OA. Methods OA was induced in the right knee joint stifle of 12-week-old male C57BL 6 mice by transforming growth factor P1 TGFp 1 injection and treadmill running for 14 days. Gait parameters were quantified by using TreadScan mechanical allodynia was evaluated with von Frey filaments and joint pathology was evaluated by scoring of macroscopic images for both cartilage erosion and periarticular fibrosis. HA or saline control was injected 1 day after TGFP1 injection but before the start of treadmill running. Results OA development in this model was accompanied by significant P enhancement of the stance and propulsion times of affected legs. HA injection but not saline injection blocked all gait changes and also protected joints from femoral cartilage erosion as well as tibial and femoral tissue fibrosis. Both HA injection and saline injection attenuated acute allodynia but the HA effect was more pronounced and prolonged than the saline injection. Conclusions We conclude that videographic gait analysis is an objective sensitive and reproducible means of monitoring joint pathology in experimental murine OA since stance time appears to correlate directly with OA severity. A single injection of HA prevents acute and prolonged gait changes .