tailieunhanh - Báo cáo y học: " Upregulated Genes In Sporadic, Idiopathic Pulmonary Arterial Hypertension"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Upregulated Genes In Sporadic, Idiopathic Pulmonary Arterial Hypertension. | Respiratory Research BioMed Central Research Open Access Upregulated Genes In Sporadic Idiopathic Pulmonary Arterial Hypertension Alasdair J Edgar 1 Matilde R Chacón2 Anne E Bishop3 Magdi H Yacoub4 and Julia M Polak3 Address Department of Craniofacial Development King s College London SE1 9RT UK 2Hospital Universitari de Tarragona Joan XXIII Unitat de Recerca C Dr. Mallafre Guash 4 43007 Tarragona Spain 3Tissue Engineering and Regenerative Medicine Centre Faculty of Medicine Imperial College London SW10 9NH UK and 4Heart Science Centre Imperial College Harefield Middlesex UB9 6JH UK Email Alasdair J Edgar - Matilde R Chacón - mrodrig@ Anne E Bishop - Magdi H Yacoub - Julia M Polak - Corresponding author Published 03 January 2006 Received 31 August 2005 Respiratory Research 2006 7 1 doi l465-992l-7-1 Accepted 03 January 2006 This article is available from http content 7 l l 2006 Edgar et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background To elucidate further the pathogenesis of sporadic idiopathic pulmonary arterial hypertension IPAH and identify potential therapeutic avenues differential gene expression in IPAH was examined by suppression subtractive hybridisation SSH . Methods Peripheral lung samples were obtained immediately after removal from patients undergoing lung transplant for IPAH without familial disease and control tissues consisted of similarly sampled pieces of donor lungs not utilised during transplantation. Pools of lung mRNA from IPAH cases containing plexiform lesions and normal donor lungs were used to generate the tester and driver cDNA .