tailieunhanh - Báo cáo y học: " Pathogenic effect of interleukin-17A in induction of Sjögren’s syndrome-like disease using adenovirus-mediated gene transfer"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Pathogenic effect of interleukin-17A in induction of Sjögren’s syndrome-like disease using adenovirus-mediated gene transfer. | Nguyen et al. Arthritis Research Therapy 2010 12 R220 http content 12 6 R220 RESEARCH ARTICLE Open Access Pathogenic effect of interleukin-17A in induction of Sjogren s syndrome-like disease using adenovirus-mediated gene transfer I ir iOd o l ldi 1 2 3 4N ncido Vito5 R moi I I dd3 ji í jr i -ì3 rrfrrr Ạ Ahirsritoi5 R Pmc-b3 4 6 Cuong Q Nguyen nunyen Iin Byung Ha Lee vvenuy C Caicamo John A oiiunni Ammon B reck Abstract Introduction Sjogren s syndrome SS involves a chronic progressive inflammation primarily of the salivary and lacrimal glands leading to decreased levels of saliva and tears resulting in dry mouth and dry eye diseases. Seminal findings regarding TH17 cell populations that secrete predominantly interleukin IL -17A have been shown to play an important role in an increasing number of autoimmune diseases including SS. In the present study we investigated the function of IL-17A on the development and onset of SS. Methods Adenovirus serotype 5 Ad5 vectors expressing either IL-17A or LacZ were infused via retrograde cannulation into the salivary glands of C57BL 6J mice between 6 and 8 weeks of age or between 15 and 17 weeks of age. The mice were characterized for SS phenotypes. Results Disease profiling indicated that SS-non-susceptible C57BL 6J mice whose salivary glands received the Ad5-IL17A vector developed a SS-like disease profile including the appearance of lymphocytic foci increased cytokine levels changes in antinuclear antibody profiles and temporal loss of saliva flow. Conclusions Induction of SS pathology by IL-17A in SS-non-susceptible mice strongly suggests that IL-17A is an important inflammatory cytokine in salivary gland dysfunction. Thus localized anti-IL17 therapy may be effective in preventing glandular dysfunction. Introduction Sjogren s syndrome SS is a chronic systemic autoimmune disease characterized most notably by development of dry eyes and dry mouth manifestations indicative of secretory dysfunction of the