tailieunhanh - Báo cáo y học: "Increased interleukin-23 receptor+ T cells in peripheral blood mononuclear cells of patients with systemic lupus erythematosu"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Increased interleukin-23 receptor+ T cells in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. | Puwipirom et al. Arthritis Research Therapy 2010 12 R215 http content 12 6 R215 RESEARCH ARTICLE Open Access Increased interleukin-23 receptor T cells in peripheral blood mononuclear cells of patients with systemic lupus erythematosus Hathaipat Puwipirom 1 2 Nattiya Hirankarn1 2 3 Pimpayao Sodsai1 Yingyos Avihingsanon1 4 Jongkonnee Wongpiyabovorn1 2 3 Tanapat Palaga2 5 Abstract Introduction Systemic lupus erythematosus SLE is an autoimmune disorder characterized by production of autoantibodies and immune complex deposition in various organs. Aberrations in the T lymphocyte compartment and dysregulated cytokine production are key features of SLE pathogenesis and disease progression. Recently the role of the interleukin IL -17 IL-23 axis in the pathogenesis of SLE has been reported. IL-23 and IL-23R are essential for expansion of pathogenic IL-17-producing T lymphocytes and have been shown to be important in the pathogenesis of lupus in animal models. Methods In this study the expression of IL-23R and IL-17 in CD4 and CD8 T lymphocytes in peripheral blood mononuclear cells PBMCs of SLE patients and control subjects were examined by flow cytometry. Twenty-nine SLE patients and 10 control subjects were recruited in this study. Patients were divided into active and inactive groups based on the SLE disease activity index SLEDAI . As another disease control population five psoriatic patients were recruited in this study. Results Percentages of both IL23R CD4 and IL-23R CD8 T cell subsets were significantly higher in freshly isolated PBMCs from both groups of SLE patients compared to control subjects P and P respectively . In addition this difference was maintained after ex vivo stimulation with plate-bound anti-CD3 CD28 antibodies P and P respectively . When the fold increase in IL-17 T cells after ex vivo stimulation for three days was compared between patients and controls SLE patients exhibited significantly higher .