tailieunhanh - Báo cáo y học: "Identification of new autoantibody specificities directed at proteins involved in the transforming growth factor b pathway in patients with systemic sclerosis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of new autoantibody specificities directed at proteins involved in the transforming growth factor b pathway in patients with systemic sclerosis. | Bussone et al. Arthritis Research Therapy 2011 13 R74 http content 13 3 R74 RESEARCH ARTICLE Open Access Identification of new autoantibody specificities directed at proteins involved in the transforming growth factor b pathway in patients with systemic sclerosis 3 3 Guillaume Bussone 1 Hanadi Dib 1 Mathieu C Tamby 1 Cedric Broussard Christian Federici Genevieve Woimant4 Luc Camoin3 Loic Guillevin5 and Luc Mouthon1 2 5 Abstract Introduction Antinuclear antibodies ANAs usually detected by indirect immunofluorescence on HEp-2 cells are identified in 90 of patients with systemic sclerosis SSc . Thus approximately 10 of SSc patients have no routinely detectable autoantibodies and for 20 to 40 of those with detectable ANAs the ANAs do not have identified specificity unidentified ANAs . In this work we aimed to identify new target autoantigens in SSc patients. Methods Using a proteomic approach combining two-dimensional electrophoresis and immunoblotting with HEp-2 cell total and enriched nuclear protein extracts as sources of autoantigens we systematically analysed autoantibodies in SSc patients. Sera from 45 SSc patients were tested in 15 pools from groups of three patients with the same phenotype. A sera pool from 12 healthy individuals was used as a control. Proteins of interest were identified by mass spectrometry and analysed using Pathway Studio software. Results We identified 974 and 832 protein spots in HEp-2 cell total and enriched nuclear protein extracts respectively. Interestingly a-enolase was recognised by immunoglobulin G IgG from all pools of patients in both extracts. Fourteen and four proteins were recognised by IgG from at least 75 of the 15 pools in total and enriched nuclear protein extracts respectively whereas 15 protein spots were specifically recognised by IgG from at least four of the ten pools from patients with unidentified ANAs. The IgG intensity for a number of antigens was higher in sera from patients .