tailieunhanh - Báo cáo y học: "RNA interference for CFTR attenuates lung fluid absorption at birth in rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "RNA interference for CFTR attenuates lung fluid absorption at birth in rats. | Respiratory Research BioMed Central Open Access RNA interference for CFTR attenuates lung fluid absorption at birth in rats Tianbo Li Shyny Koshy and Hans G Folkesson Address Department of Integrative Medical Sciences Northeastern Ohio Universities Colleges of Medicine and Pharmacy Rootstown OH 442720095 USA Email Tianbo Li - Shyny Koshy - skoshy@ Hans G Folkesson - hgfolkes@ Corresponding author Published 24 July 2008 Received 6 February 2008 Respiratory Research 2008 9 55 doi 1465-9921 -9-55 Accepted 24 July 2008 This article is available from http content 9 1 55 2008 Li et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Small interfering RNA siRNA against aENaC a-subunit of the epithelial Na channel and CFTR cystic fibrosis transmembrane conductance regulator was used to explore ENaC and CTFR function in newborn rat lungs. Methods Twenty-four hours after trans-thoracic intrapulmonary ttip injection of siRNAgenerating plasmid DNA pSi-0 pSi-4 or pSi-C2 we measured CFTR and ENaC expression extravascular lung water and mortality. Results aENaC and CFTR mRNA and protein decreased by 80 and 85 respectively following aENaC and CFTR silencing. Extravascular lung water and mortality increased after aENaC and CFTR-silencing. In pSi-C2-transfected isolated DLE cells there were attenuated CFTR mRNA and protein. In pSi-4-transfected DLE cells aENaC mRNA and protein were both reduced. Interestingly CFTR-silencing also reduced aENaC mRNA and protein. aENaC silencing on the other hand only slightly reduced CFTR mRNA and protein. Conclusion Thus ENaC and CFTR are both involved in the fluid secretion to absorption conversion around at birth. .

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