tailieunhanh - Báo cáo y học: " The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures. | Respiratory Research BioMed Central Research Open Access The role of IFN-y in regulation of IFN-y-inducible protein 10 IP-10 expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures Maria Torvinen Hinnah Campwala and Iain Kilty Address Pfizer Global R D Dep. Allergy and Respiratory 500 Pfizer Ltd. Sandwich Kent CT13 9NJ UK Email Maria Torvinen - torvinenm@ Hinnah Campwala - Iain Kilty - Corresponding author Published 8 November 2007 Received 5 December 2006 Respiratory Research 2007 8 80 doi 1465-9921 -8-80 Accepted 8 November 2007 This article is available from http content 8 1 80 2007 Torvinen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Interferons play a critical role in regulating both the innate and adaptive immune responses. Previous reports have shown increased levels of IFN-y IFN-y-inducing IL-12 and IFN-y-inducible chemokine IP-10 in patients with chronic obstructive pulmonary disease COPD . Methods The present study focuses on the regulation of the IP-10 secretion in co-cultures of lung epithelial cells and peripheral blood mononuclear cells PBMCs . Results No IP-10 secretion was detected in cells cultured alone whereas a significant increase in IP-10 levels was observed in epithelial cell PBMC co-cultures. Furthermore the results show that interactions between lung epithelial cells lymphocytes and monocytes are needed for basal IP-10 secretion. Interestingly we have also shown that incubation with IL-12 can induce an IFN-y independent increase in IP-10 levels in co-cultures. Furthermore inhibition studies supported the suggestion that different intracellular .