tailieunhanh - Báo cáo y học: "Reduced influenza viral neutralizing activity of natural human trimers of surfactant protein D"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Reduced influenza viral neutralizing activity of natural human trimers of surfactant protein D. | Respiratory Research BioMed Central Research Open Access Reduced influenza viral neutralizing activity of natural human trimers of surfactant protein D Kevan L Hartshorn 1 Mitchell R White1 Tesfaldet Tecle1 Ida Tornoe3 Grith L Sorensen3 Erika C Crouch2 and Uffe Holmskov3 Address 1Boston University School of Medicine Department of Medicine Boston MA USA 2Washington University School of Medicine Department of Pathology and Immunology St. Louis MO USA and 3Medical Biotechnology Center University of Southern Denmark Odense Denmark Email Kevan L Hartshorn - khartsho@ Mitchell R White - mitchw@ Tesfaldet Tecle - ttecle@ Ida Tornoe - ITornoe@ Grith L Sorensen - glsorensen@ Erika C Crouch - crouch@ Uffe Holmskov- uholmskov@ Corresponding author Published 5 February 2007 Received 23 June 2006 Respiratory Research 2007 8 9 doi 1465-9921-8-9 Accepted 5 February 2007 This article is available from http content 8 1 9 2007 Hartshorn et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Surfactant protein D SP-D plays important roles in innate host defense against influenza A virus IAV infection. Common human polymorphisms of SP-D have been found in many human populations and associated with increased risk of certain infections. We recently reported that the Thr Thr 11 form of SP-D is associated with low serum levels and assembles predominantly as trimers as opposed to the more common multimeric forms of SP-D. Methods Preliminary experiments were done to establish the effects of different monoclonal antibodies against SP-D on ability of SP-D to bind to or neutralize the virus. We then purified natural human trimeric and .