tailieunhanh - Báo cáo y học: "Invasive and noninvasive methods for studying pulmonary function in mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Invasive and noninvasive methods for studying pulmonary function in mice. | Respiratory Research BioMed Central Review Open Access Invasive and noninvasive methods for studying pulmonary function in mice Thomas Glaab1 Christian Taube1 Armin Braun 2 and Wayne Mitzner3 Address Department of Pulmonary Medicine III. Medical Clinic Johannes Gutenberg-University Mainz Germany 2Fraunhofer Institute of Toxicology and Experimental Medicine ITEM Hannover Germany and 3Division of Physiology Bloomberg School of Public Health Johns Hopkins University Baltimore Maryland 21205 USA Email Thomas Glaab - thomasglaab@ Christian Taube - taube@ Armin Braun - braun@ Wayne Mitzner - wmitzner@ Corresponding author Published 14 September 2007 Received 12 April 2007 Accepted 14 September 2007 Respiratory Research 2007 8 63 doi 1465-9921 -8-63 This article is available from http content 8 1 63 2007 Glaab et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The widespread use of genetically altered mouse models of experimental asthma has stimulated the development of lung function techniques in vivo to characterize the functional results of genetic manipulations. Here we describe various classical and recent methods of measuring airway responsiveness in vivo including both invasive methodologies in anesthetized intubated mice repetitive non-repetitive assessment of pulmonary resistance RL and dynamic compliance Cdyn measurement of low-frequency forced oscillations LFOT and noninvasive technologies in conscious animals head-out body plethysmography barometric whole-body plethysmography . Outlined are the technical principles validation and applications as well as the strengths and weaknesses of each methodology. Reviewed is the