tailieunhanh - Báo cáo y học: "Perforin, granzyme B, and FasL expression by peripheral blood T lymphocytes in emphysema"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Perforin, granzyme B, and FasL expression by peripheral blood T lymphocytes in emphysema. | Respiratory Research BioMed Central Research Open Access Perforin granzyme B and FasL expression by peripheral blood T lymphocytes in emphysema Mathieu C Morissette Julie Parent and Julie Milot Address Hôpital Laval Institut de cardiologie et de pneumologie Québec Qc Canada Email Mathieu C Morissette - Julie Parent - Julie Milot - Corresponding author Published 6 September 2007 Received 18 April 2007 Respiratory Research 2007 8 62 doi 1465-9921 -8-62 Accepted 6 September 2007 This article is available from http content 8 1 62 2007 Morissette et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background It is generally accepted that emphysematous lungs are characterized by an increase in the numbers of neutrophils macrophages and CD8 T lymphocytes the lasts having increased cytotoxic activity. Because systemic inflammation is also a component of emphysema we hypothesize that peripheral CD8 T lymphocytes of emphysematous smokers who show evidence of systemic inflammation will have higher expression of cytotoxic molecules. Methods We assessed parameters of systemic inflammation in normal individuals smokers or non-smokers and in emphysematous subjects with an active smoking history by measuring serum interleukine-6 C-reactive protein and tumor necrosis factor. Expression of perforin granzyme B and FasL protein by CD8 T lymphocytes CD4 T lymphocytes and natural killer cells were assessed by flow cytometry while perforin granzyme B and FasL mRNA expression were measured on purified systemic CD8 T lymphocytes by real-time PCR. Results Emphysematous smokers had higher levels of serum interleukine-6