tailieunhanh - Báo cáo y học: "Antisense oligonucleotide inhibition of Heat Shock Protein (HSP) 47 improves bleomycin-induced pulmonary fibrosis in rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Antisense oligonucleotide inhibition of Heat Shock Protein (HSP) 47 improves bleomycin-induced pulmonary fibrosis in rats. | Respiratory Research BioMed Central Research Antisense oligonucleotide inhibition of Heat Shock Protein HSP 47 improves bleomycin-induced pulmonary fibrosis in rats Satoshi Hagiwara Hideo Iwasaka Shigekiyo Matsumoto and Takayuki Noguchi Open Access Address Department of Brain and Nerve Science Anesthesiology Oita University Faculty of Medicine 1-1 Idaigaoka-Hasamamachi-Yufu City-Oita 879-5593 Japan Email Satoshi Hagiwara - saku@ Hideo Iwasaka - hiwasaka@ Shigekiyo Matsumoto - SIGEKIYO@ Takayuki Noguchi - TNOGU@ Corresponding author Published 15 May 2007 Respiratory Research 2007 8 37 doi l465-992l-8-37 Received 10 February 2007 Accepted 15 May 2007 This article is available from http content 8 l 37 2007 Hagiwara et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background The most common pathologic form of pulmonary fibrosis arises from excessive deposition of extracellular matrix proteins such as collagen. The 47 kDa heat shock protein 47 HSP47 is a collagen-specific molecular chaperone that has been shown to play a major role during the processing and or secretion of procollagen. Objectives To determine whether inhibition of HSP47 could have beneficial effects in mitigating bleomycin-induced pulmonary fibrosis in rats. Methods All experiments were performed with 250-300 g male Wistar rats. Animals were randomly divided into five experimental groups that were administered l saline alone 2 bleomycin alone 3 antisense HSP47 oligonucleotides alone 4 bleomycin antisense HSP47 oligonucleotides and 5 bleomycin sense control .