tailieunhanh - Báo cáo y học: " Genomic analysis of human lung fibroblasts exposed to vanadium pentoxide to identify candidate genes for occupational bronchitis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Genomic analysis of human lung fibroblasts exposed to vanadium pentoxide to identify candidate genes for occupational bronchitis. | Respiratory Research BioMed Central Research Open Access Genomic analysis of human lung fibroblasts exposed to vanadium pentoxide to identify candidate genes for occupational bronchitis Jennifer L Ingram Aurita Antao-Menezes Elizabeth A Turpin Duncan G Wallace James B Mangum Linda J Pluta Russell S Thomas and James C Bonner Address The Hamner Institutes for Health Sciences Research Triangle Park North Carolina 27709 USA Email Jennifer L Ingram - Aurita Antao-Menezes - amenezes@ Elizabeth A Turpin - eturpin@ Duncan G Wallace - wallace@ James B Mangum - Linda J Pluta - lpluta@ Russell S Thomas - rthomas@ James C Bonner - jbonner@ Corresponding author Published 25 April 2007 Received 5 December 2006 Respiratory Research 2007 8 34 doi 1465-9921 -8-34 Accepted 25 April 2007 This article is available from http content 8 1 34 2007 Ingram et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Exposure to vanadium pentoxide V2O5 is a cause of occupational bronchitis. We evaluated gene expression profiles in cultured human lung fibroblasts exposed to V2O5 in vitro in order to identify candidate genes that could play a role in inflammation fibrosis and repair during the pathogenesis of V2O5-induced bronchitis. Methods Normal human lung fibroblasts were exposed to V2O5 in a time course experiment. Gene expression was measured at various time points over a 24 hr period using the Affymetrix Human Genome U133A Array. Selected genes that were significantly changed in the microarray experiment were validated by RT-PCR. Results V2O5 altered more than 1 400 genes of which 300 were induced .