tailieunhanh - Báo cáo y học: " The role of γδ T cells in airway epithelial injury and bronchial responsiveness after chlorine gas exposure in mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " The role of γδ T cells in airway epithelial injury and bronchial responsiveness after chlorine gas exposure in mice. | Respiratory Research BioMed Central Research Open Access The role of yS T cells in airway epithelial injury and bronchial responsiveness after chlorine gas exposure in mice Hossein Koohsari Meiyo Tamaoka Holly R Campbell and James G Martin Address Meakins-Christie Laboratories McGill University Montreal QC Canada Email Hossein Koohsari - hkoohsari@ Meiyo Tamaoka - meiyou@ Holly R Campbell - holly@ James G Martin - Corresponding author Published 7 March 2007 Received 10 November 2006 Respiratory Research 2007 8 21 doi 1465-9921 -8-21 Accepted 7 March 2007 This article is available from http content 8 1 21 2007 Koohsari et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Acute exposure to chlorine Cl2 gas causes epithelial injury and airway dysfunction. yS T cells are present in the mucosal surface of the airways and may contribute to the injury repair response of the epithelium. Methods C57Bl 6J wild type and TCR-S- - mice exposed to Cl2 400 ppm for 5 minutes underwent measurements of airway responses to . methacholine MCh at 1 3 and 5 days after exposure. Bronchoalveolar lavage was performed to determine epithelial and leukocyte counts and protein content. Tissue repair was assessed by proliferating cell nuclear antigen PCNA immunoreactivity and by expression of keratinocyte growth factor KGF mRNA by real-time PCR. Results Wild type mice developed a greater degree of airway hyperresponsiveness to MCh at 1 day post exposure to Cl2 compared with TCR-S- - mice. Epithelial cell counts in BAL after Cl2 exposure were greater in TCR-S- - mice but macrophages showed a later peak and granulocyte numbers were lower .