tailieunhanh - Báo cáo y học: "Immediate determination of ACPA and rheumatoid factor - a novel point of care test for detection of anti-MCV antibodies and rheumatoid factor using a lateral-flow immunoassay"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Immediate determination of ACPA and rheumatoid factor - a novel point of care test for detection of anti-MCV antibodies and rheumatoid factor using a lateral-flow immunoassay. | Renger et al. Arthritis Research Therapy 2010 12 R120 http content 12 3 R120 RESEARCH ARTICLE Open Access Immediate determination of ACPA and rheumatoid factor - a novel point of care test for detection of anti-MCV antibodies and rheumatoid factor using a lateral-flow immunoassay Franziska Renger 1 Holger Bang2 Eugen Feist1 Gert Fredenhagen2 Alexander Natusch3 Marina Backhaus1 Gerd-R Burmester1 and Karl Egerer1 Abstract Introduction Autoantibodies against mutated and citrullinated vimentin MCV represent a novel diagnostic marker for rheumatoid arthritis RA . Recently an increased sensitivity for anti-MCV compared to autoantibodies against cyclic citrullinated peptides anti-CCP2 was shown in cohorts of patients with early RA and established disease. The aim of this study was to develop and evaluate a point of care test POCT for detection of anti-MCV antibodies immediately at the first visit or at the bed side. Methods A lateral-flow immunoassay was developed for simultaneous detection of anti-MCV antibodies and rheumatoid factor RF-IgG and evaluated in a prospective setting. Analyses were performed from whole blood samples of patients with seropositive RA n 108 seronegative RA as well as other rheumatic disorders n 122 and healthy blood donors n 200 and compared to detection via ELISA. Results Using the POCT anti-MCV antibodies were detected in and RF-IgG in of patients with RA. Specificity was for anti-MCV antibodies and for RF-IgG. Compared to ELISA s results POCT sensitivity was for anti-MCV and for RF-IgG specificity was and respectively. Conclusions This POCT for detection of anti-MCV antibodies and RF-IgG provides high specificity for the diagnosis of RA and is useful in clinical practice due to its simplicity and its reliable performance. This test can greatly improve a timely management of RA and may help in screening patients with suspected RA in non-specialized settings prompting early .