tailieunhanh - Báo cáo y học: "Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection. | Rutgers et al. Arthritis Research Therapy 2010 12 R114 http content 12 3 R114 RESEARCH ARTICLE Open Access Cytokine profile of autologous conditioned serum for treatment of osteoarthritis in vitro effects on cartilage metabolism and intra-articular levels after injection Marijn Rutgers 1 Daniel BF Saris1 Wouter JA Dhert1 2 and Laura B Creemers 1 Abstract Introduction Intraarticular administration of autologous conditioned serum ACS recently demonstrated some clinical effectiveness in treatment of osteoarthritis OA . The current study aims to evaluate the in vitro effects of ACS on cartilage proteoglycan PG metabolism its composition and the effects on synovial fluid SF cytokine levels following intraarticular ACS administration. Methods The effect of conditioned serum on PG metabolism of cultured OA cartilage explants was compared to unconditioned serum. The effect of serum conditioning on levels of interleukin-1beta IL-1P IL-4 IL-6 IL-10 IL-13 interferon gamma IFN-y tumor necrosis factor alpha TNF-a osteoprotegerin OPG oncostatin M OSM interleukin-1 receptor IL-1ra and transforming growth factor beta TGF-P were measured by multiplex ELISA. As TNF-a levels were found to be increased in conditioned serum the effect of TNF-a inhibition by etanercept on PG metabolism was studied in cartilage explants cultured in the presence of conditioned serum. Furthermore cytokine levels in SF were measured three days after intraarticular ACS injection in OA patients to verify their retention time in the joint space. Results PG metabolism was not different in the presence of conditioned serum compared to unconditioned serum. Levels of the anti-inflammatory cytokines IL-1ra TGF-p IL-10 as well as of pro-inflammatory cytokines IL-1 p IL-6 TNF-a and OSM were increased. IL-4 IL-13 and IFN-Y levels remained similar while OPG levels decreased. TNF-a inhibition did not influence PG metabolism in cartilage explant culture in the presence of condtioned serum. Although