tailieunhanh - Báo cáo y học: "Functional significance of nerve growth factor and its receptor (TrkA) in inflammatory arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Functional significance of nerve growth factor and its receptor (TrkA) in inflammatory arthritis. | Raychaudhuri and Raychaudhuri Arthritis Research Therapy 2010 12 404 http content 12 3 404 LETTER Functional significance of nerve growth factor and its receptor TrkA in inflammatory arthritis Smriti K Raychaudhuri and Siba P Raychaudhuri See related research by Barthel etal. http content 11 3 R82 We read the article by Barthel and colleagues 1 in this issue of Arthritis Research Therapy with great interest. They reported that neurotrophin receptors ligands and specifically nerve growth factor NGF and NGF receptor NGF-R TrkA and p75 are expressed in synovial fluid SF cells and synovial tissue ST of patients with rheumatoid arthritis RA or spondyloarthritis SpA . The authors also looked for the cellular source of NGF and demonstrated that the T cells and monocytes macro-phages derived from SF of patients with RA SpA are enriched with NGF. Furthermore they reported that ST-derived fibroblast-like synoviocytes FLSs did not produce NGF in vitro. In a recent publication we observed similarly that NGF levels in SF were significantly higher in patients with psoriatic arthritis PsA pg mL or RA 120 35 pg mL than in patients with osteoarthritis OA 30 6 pg mL 2 . However in regard to the source of NGF we observed that FLSs produced a significant amount of NGF. Here we would like to share our observations about the NGF TrkA system in human FLSs and its function. Synovial biopsies from patients with meniscal injury without any other joint diseases or PsA OA or RA were collected. FLSs were isolated and examined for NGF NGF-R expression in accordance with our standardized protocols 2 . Using a highly sensitive enzyme-linked immunosorbent assay ELISA for human NGF NGFEmax assay Promega Corporation Madison WI USA and Hi-D fluorescence-activated cell sorting analyses we observed that under basal conditions FLSs from healthy individuals express low levels of NGF TrkA. However there was a marked upregulation of NGF and TrkA in .