tailieunhanh - Báo cáo khoa học: In vitro characterization of Synechocystis CYP120A1 revealed the first nonanimal retinoic acid hydroxylase

Retinoids are C20 apocarotenoids that have various important functions in metazoans. In addition, several findings suggest their occurrence in eubac-teria, including cyanobacteria. It has been shown that the Synechocystis cytochrome P450 enzyme CYP120A1 is a retinoic acid-binding polypeptide. In this work, we determined the reaction catalyzed by CYP120A1 and investigated its substrate specificity in vitro. | In vitro characterization of Synechocystis CYP120A1 revealed the first nonanimal retinoic acid hydroxylase Adrian AIHpr1 Pntnr Rinlnr2 Plan in In A nrolr-Rniohhart3 nnd Ralim Ạ I - R a h 1111 Ilan cl del yiei allieic CICI eiuail all aill a 1 Faculty of Biology Institute of Biology II Albert-Ludwigs University of Freiburg Germany 2 Department of Chemistry and Biochemistry NMR Laboratory University of Bern Switzerland 3 Institute of Plant Molecular Biology CNRS-UPR2357 University of Strasbourg France Keywords b-apo-13-carotenone carotenoid cleavage cyanobacteria cytochrome P450 retinoic acid Correspondence S. Al-Babili Albert-Ludwigs University of Freiburg Institute for Biology II Cell Biology Schaenzlestr. 1 D-79104 Freiburg Germany Fax 49 761 203 2675 Tel 49 761 203 8454 E-mail Received 25 May 2009 revised 26 June 2009 accepted 21 July 2009 doi Retinoids are C20 apocarotenoids that have various important functions in metazoans. In addition several findings suggest their occurrence in eubac-teria including cyanobacteria. It has been shown that the Synechocystis cytochrome P450 enzyme CYP120A1 is a retinoic acid-binding polypeptide. In this work we determined the reaction catalyzed by CYP120A1 and investigated its substrate specificity in vitro. CYP120A1-containing microsomes generated in yeast converted all-trans-retinoic acid into a compound exhibiting higher polarity in HPLC analysis. Liquid chromatography-MS analysis suggested the introduction of a single hydroxyl group and NMR analysis of the purified product revealed C16 or C17 as the reaction site. Incubations with cis-retinoic acids retinal 3 R -OH-retinal retinol b-apo-13-carotenone Cis and b-apo-14 -carotenal C22 resulted in the formation of the corresponding hydroxyl derivatives as suggested by HPLC and liquid chromatography-MS analyses. Comparisons of the relative product amounts revealed the highest conversion rate for .

• Báo cáo khoa học
• scientific reports
• thesis report
• medical report
• Crystal structure
• medical analysis
• biochemical studies
• Zizyphus mauritiana Lamk
• Biochemical constitutes
• Ber selection 1
• Studies on the pattern
• Pattern of changes biochemical constitutes
• Studies on Tikka disease
• Tikka disease
• Sowing dates
• Biochemical parameters of groundnut
• Biochemical parameters
• Studies on biochemical changes
• Biochemical changes
• Cell wall degrading enzymes
• Papaya fruit inoculated
• Colletotrichum demetium
• Vindhyan zone
• Haemato biochemical profile
• Haemato biochemical profile of goats
• Goats in Vindhyan region
• Biochemical variables
• Diaphragmatic hernia
• Various NSAIDS protocols affected
• Buffaloes treated
• Biochemical composition
• Reducing and Non reducing sugar
• Non reducing sugar
• Biochemical composition of various tomato
• Various tomato (Solanum lycopersicum L )
• Report medicine
• traditional medicine
• environmental medicine
• Hepatobiliary disorders
• Serum biochemistry
• Hemato biochemical studies
• Hepatobiliary disorders in dogs
• Elevated total leucocyte
• Agricultural science
• Physiological and biochemical studies
• Wheat plants by application
• Amino acids and yeast extract
• Physio chemical
• Water relations
• Lucknow 49
• Ascarbic acid
• Different cultivars
• Guava under eastern
• Biochemical studies on adverse health
• Adverse health effects of arsenic
• Chromium in buffaloes
• Level of arsenic and chromium
• Heavy metals in buffaloes
• PyometraCEH complex
• Haemato biochemical evaluation
• Bitches affected
• Cystic endometrial hyperplasia pyometra complex
• Haemato biochemical studies
• Sloth bears
• HematoBiochemical changes
• Biochemical values
• Hemato biochemical changes
• Xylazine Zolazepam
• Sahiwal cows
• Blood metabolites
• Complete lactation in Sahiwal cows
• breast cancer
• medicine
• Hematopoietic
• medical knowledge