tailieunhanh - NEW PERSPECTIVES IN BIOSENSORS TECHNOLOGY AND APPLICATIONS_2

Tham khảo sách 'new perspectives in biosensors technology and applications_2', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 10 Mathematical Modeling of Biosensors Enzyme-substrate Interaction and Biomolecular Interaction A. Meena A. Eswari and L. Rajendran Department of Mathematics The Madura College Madurai- 625011 Tamilnadu India 1. Introduction The vast majority of chemical transformations inside cells are carried out by proteins called enzymes. Enzymes accelerate the rate of chemical reactions both forward and backward without being consumed in the process and tend to be very selective with a particular enzyme accelerating only a specific reaction. Enzymes are important in regulating biological processes for example as activators or inhibitors in a reaction. To understand the role of enzyme kinetics the researcher has to study the rates of reactions the temporal behaviours of the various reactants and the conditions which influence the enzyme kinetics. Introduction with a mathematical bent is given in the books by Rubinow 1975 Murray 1989 Segel 1980 and Roberts 1977 . Biosensors are analytical devices made up of a combination of a specific biological element usually an enzyme that recognizes a specific analyte substrate and the transducer that translates the biorecognition event into an electrical signal Tuner et al. 1987 Scheller et al. 1992 . Amperometric biosensors may utilize one two three or multi enzymes Kulys 1981 . The classical example of mono enzyme biosensor might be the biosensor that contains membrane with immobilized glucose oxidase. The glucose oxidase specifically oxidizes glucose to hydrogen peroxide that is determined ampero-metrically on platinum electrode Kulys 1981 . The amperometric biosensors measure the current that arises on a working electrode by direct electrochemical oxidation or reduction of the biochemical reaction product. The current is proportionate to the concentration of the target analyte. The biosensors are widely used in clinical diagnostics environment monitoring food analysis and drug detection because they are reliable highly sensitive and .

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