tailieunhanh - Báo cáo hóa học: " Research Article Genome-Wide Analysis of Intergenic Regions of Mycobacterium tuberculosis H37Rv Using Affymetrix GeneChips"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Research Article Genome-Wide Analysis of Intergenic Regions of Mycobacterium tuberculosis H37Rv Using Affymetrix GeneChips | Hindawi Publishing Corporation EURASIP Journal on Bioinformatics and Systems Biology Volume 2007 Article ID 23054 7 pages doi 2007 23054 Research Article Genome-Wide Analysis of Intergenic Regions of Mycobacterium tuberculosis H37Rv Using Affymetrix GeneChips Li M. Fu1 and Thomas M. Shinnick2 1 Pacific Tuberculosis and Cancer Research Organization 8 Corporate Park Suite 300 Irvine CA 92606 USA 2 Centers for Disease Control and Prevention Atlanta GA 30333 USA Received 24 April 2007 Accepted 14 August 2007 Recommended by Z. Jane Wang Sequencing the complete genome of Mycobacterium tuberculosis H37Rv is a major milestone in the genome project and it sheds new light in our fight with tuberculosis. The genome contains around 4000 genes protein-coding sequences in the original genome annotation. A subsequent reannotation of the genome has added 80 more genes. However we have found that the inter-genic regions can exhibit expression signals as evidenced by microarray hybridization. It is then reasonable to suspect that there are unidentified genes in these regions. We conducted a genome-wide analysis using the Affymetrix GeneChip to explore genes contained in the intergenic sequences of the M. tuberculosis H37Rv genome. A working criterion for potential protein-coding genes was based on bioinformatics consisting of the gene structure protein coding potential and presence of ortholog evidence. The bioinformatics criteria in conjunction with transcriptional evidence revealed potential genes with a specific function such as a DNA-binding protein in the CopG family and a nickle binding GTPase as well as hypothetical proteins that had not been reported in the H37Rv genome. This study further demonstrated that microarray-based transcriptional evidence would facilitate genome-wide gene finding and is also the first report concerning intergenic expression in M. tuberculosis genome. Copyright 2007 L. M. Fu and T. M. Shinnick. This is an open access article distributed .