tailieunhanh - Báo cáo y học: "Citrullinated vimentin as an important antigen in immune complexes from synovial fluid of rheumatoid arthritis patients with antibodies against citrullinated proteins"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Citrullinated vimentin as an important antigen in immune complexes from synovial fluid of rheumatoid arthritis patients with antibodies against citrullinated proteins. | Van Steendam et al. Arthritis Research Therapy 2010 12 R1 32 http content 12 4 R132 RESEARCH ARTICLE Open Access Citru llinated vimentin as an important antigen in immune complexes from synovial fluid of rheumatoid arthritis patients with antibodies against citrullinated proteins Katleen Van Steendam 1 Kelly Tilleman1 Marlies De Ceuleneer1 Filip De Keyser2 Dirk Elewaut2 and Dieter Deforce 1 Abstract Introduction Rheumatoid arthritis RA is an inflammatory disease which results in destruction of the joint. The presence of immune complexes IC in serum and synovial fluid of RA patients might contribute to this articular damage through different mechanisms such as complement activation. Therefore identification of the antigens from these IC is important to gain more insight into the pathogenesis of RA. Since RA patients have antibodies against citrullinated proteins ACPA in their serum and synovial fluid SF and since elevated levels of citrullinated proteins are detected in the joints of RA patients citrullinated antigens are possibly present in IC from RA patients. Methods IC from serum of healthy persons serum of RA patients and IC from synovial fluid of RA patients and Spondyloarthropathy SpA patients were isolated by immunoprecipitation. Identification of the antigens was performed by SDS-PAGE mass spectrometry and immunodetection. The presence of citrullinated proteins was evaluated by anti-modified citrulline AMC staining. Results Circulating IC in the serum of RA patients and healthy controls contain fibrinogen and fibronectin both in a non-citrullinated form. Additionally in IC isolated from RA SF fibrinogeny and vimentin were identified as well. More importantly vimentin and a minor portion of fibrinogen were found to be citrullinated in the isolated complexes. Moreover these citrullinated antigens were only found in ACPA patients. No citrullinated antigens were found in IC from SF of SpA patients. Conclusions Citrullinated fibrinogen and

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