tailieunhanh - Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment. | Buhrmann et al. Arthritis Research Therapy 2010 12 R127 http content 12 4 R127 RESEARCH ARTICLE Open Access Curcumin mediated suppression of nuclear factor-KB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment Constanze Buhrmann 1 Ali Mobasheri2 Ulrike Matis3 and Mehdi Shakibaei 1 Abstract Introduction Osteoarthritis OA and rheumatoid arthritis RA are characterised by joint inflammation and cartilage degradation. Although mesenchymal stem cell MSC -like progenitors are resident in the superficial zone of articular cartilage damaged tissue does not possess the capacity for regeneration. The high levels of pro-inflammatory cytokines present in OA RA joints may impede the chondrogenic differentiation of these progenitors. Interleukin IL -1p activates the transcription factor nuclear factor-KB NF-kB which in turn activates proteins involved in matrix degradation inflammation and apoptosis. Curcumin is a phytochemical capable of inhibiting IL-1 p-induced activation of NF-kB and expression of apoptotic and pro-inflammatory genes in chondrocytes. Therefore the aim of the present study was to evaluate the influence of curcumin on IL-ip-induced NF-kB signalling pathway in MSCs during chondrogenic differentiation. Methods MSCs were either cultured in a ratio of 1 1 with primary chondrocytes in high-density culture or cultured alone in monolayer with without curcumin and or IL-1p. Results We demonstrate that although curcumin alone does not have chondrogenic effects on MSCs it inhibits IL-1P-induced activation of NF-kB activation of caspase-3 and cyclooxygenase-2 in MSCs time and concentration dependently as it does in chondrocytes. In IL-1P stimulated co-cultures four-hour pre-treatment with curcumin significantly enhanced the production of collagen type II cartilage specific proteoglycans CSPGs p1-integrin as well as activating MAPKinase signaling and suppressing caspase-3 and .