tailieunhanh - Báo cáo y học: " Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis: implications for pathogenesis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis: implications for pathogenesis. | Hinze et al. Arthritis Research Therapy 2010 12 R123 http content 12 3 R123 RESEARCH ARTICLE Open Access Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis implications for pathogenesis 1 12 3 Claas H Hinze Ndate Fall Sherry Thornton Jun Q Mo Bruce J Aronow Gerlinde Layh-Schmitt Thomas A Griffin 1 Susan D Thompson1 Robert A Colbert1 5 David N Glass1 Michael G Barnes1 Alexei A Grom1 Abstract Introduction Previous observations suggest that active systemic juvenile idiopathic arthritis sJIA is associated with a prominent erythropoiesis gene-expression signature. The aim of this study was to determine the association of this signature with peripheral blood mononuclear cell PBMC subpopulations and its specificity for sJIA as compared with related conditions. Methods The 199 patients with JIA 23 sJIA and 176 non-sJIA and 38 controls were studied. PBMCs were isolated and analyzed for multiple surface antigens with flow cytometry and for gene-expression profiles. The proportions of different PBMC subpopulations were compared among sJIA non-sJIA patients and controls and subsequently correlated with the strength of the erythropoiesis signature. Additional gene-expression data from patients with familial hemophagocytic lymphohistiocytosis FHLH and from a published sJIA cohort were analyzed to determine whether the erythropoiesis signature was present. Results Patients with sJIA had significantly increased proportions of immature cell populations including CD34 cells correlating highly with the strength of the erythropoiesis signature. The erythropoiesis signature strongly overlapped with the gene-expression pattern in purified immature erythroid precursors. The expansion of immature cells was most prominently seen in patients with sJIA and anemia even in the absence of reticulocytosis. Patients with non-sJIA and anemia did not exhibit the erythropoiesis signature. The .